AI Article Synopsis
- The study presents a simplified Mycobacterial Growth Inhibition Assay (MGIA) to evaluate vaccine effectiveness in mice against Mycobacterium tuberculosis.
- It involves infecting splenocytes from vaccinated mice and measuring the growth of bacteria using Mycobacterial Growth Indicator Tubes (MGIT).
- The results indicate that the level of immunogenicity detected through this assay aligns with the level of protection provided by the vaccine.
Article Abstract
We describe a simplified Mycobacterial Growth Inhibition Assay (MGIA) for pre-clinical assessment of vaccine-mediated protection in mice. The assay is accomplished by directly infecting splenocytes from vaccinated mice with Mycobacterium tuberculosis and quantifying mycobacteria using Mycobacterial Growth Indicator Tubes (MGIT). Vaccine-mediated immunogenicity detected by this assay correlated with protection.
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| http://dx.doi.org/10.1016/j.mimet.2016.09.010 | DOI Listing |
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