Cell-derived exosomes are leading candidates for in vivo drug delivery carriers. In particular, exosomes derived from 293T cells are used most frequently, although exosome dosing has varied greatly among studies. Considering their biological origin, it is crucial to characterize the molecular composition of exosomes if large doses are to be administered in clinical settings. In this study, we present the first comprehensive analysis of the protein, messenger RNA and microRNA profiles of 293T cell-derived exosomes; then, we characterized these data using Gene Ontology annotation and Kyoto Encyclopedia for Genes and Genomes pathway analysis. Our study will provide the basis for the selection of 293T cell-derived exosome drug delivery systems. Profiling the exosomal signatures of 293T cells will lead to a better understanding of 293T exosome biology and will aid in the identification of any harmful factors in exosomes that could cause adverse clinical effects.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5029934 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0163043 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
Mesenchymal stem cell-derived exosome and liposome hybrids as transfection nanocarriers of Cas9-GFP plasmid to HEK293T cells.
PLoS One
January 2025
Department of Regenerative Medicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Exosomes are natural membrane-enclosed nanovesicles (30-150 nm) involved in cell-cell communication. Recently, they have garnered considerable interest as nanocarriers for the controlled transfer of therapeutic agents to cells. Here, exosomes were derived from bone marrow mesenchymal stem cells using three different isolation methods.
View Article and Find Full Text PDFCD7-targeting pro-apoptotic extracellular vesicles: A novel approach for T-cell haematological malignancy therapy.
J Extracell Vesicles
December 2024
Department of Orthopaedics of the Second Affiliated Hospital and Institute of Immunology, Zhejiang University School of Medicine, Hangzhou, China.
T-cell haematological malignancies progress rapidly and have a high mortality rate and effective treatments are still lacking. Here, we developed a drug delivery system utilizing 293T cell-derived extracellular vesicles (EVs) modified with an anti-CD7 single-chain variable fragment (αCD7/EVs). Given the challenges of chemotherapy resistance in patients with T-cell malignancy, we selected cytochrome C (CytC) and Bcl2 siRNA (siBcl2) as therapeutic agents and loaded them into αCD7/EVs (αCD7/EVs/CytC/siBcl2).
View Article and Find Full Text PDFEnhanced Gαq Signaling in -deficient Cells Is Required for Their Neoplastic Behavior.
Am J Respir Cell Mol Biol
November 2024
Research Institute of the McGill University Health Centre, Respiratory Program and Meakins-Christie Laboratories, Montreal, Quebec, Canada.
Article Synopsis
- Inherited or sporadic loss of a specific gene can lead to pulmonary lymphangioleiomyomatosis (LAM), a rare lung disease caused by tumor nodules that display characteristics of neural crest and smooth muscle cells.
- The abnormal growth of these "LAM cells" is linked to increased activity of the mTORC1 protein, which is typically regulated by the TSC1-TSC2 protein complex; while rapamycin slows LAM progression, it does not eliminate the disease, suggesting other processes are involved.
- Recent studies have identified G-protein coupled urotensin-II receptor (UT) signaling as a key player in LAM's cancer-related signaling, revealing that enhanced signaling through UT promotes harmful cell behaviors in
Checkpoint Kinase 1 Stimulates Endogenous Cardiomyocyte Renewal and Cardiac Repair by Binding to Pyruvate Kinase Isoform M2 C-Domain and Activating Cardiac Metabolic Reprogramming in a Porcine Model of Myocardial Ischemia/Reperfusion Injury.
J Am Heart Assoc
July 2024
Department of Cardiology The First Affiliated Hospital of Nanjing Medical University Nanjing China.
Article Synopsis
- The study investigates the effects of rhCHK1, a recombinant protein, on improving heart cell survival and growth after ischemia/reperfusion injury in pigs, with findings suggesting enhanced cardiac repair and function.
- Researchers found that injecting rhCHK1 not only stimulated heart cell division and reduced inflammation within days but also improved overall heart function and minimized damage over a month post-injury.
- The mechanism behind these effects involves CHK1 binding to and activating specific sites on the PKM2 protein, leading to changes in metabolism that support heart cell renewal and repair processes.
[Tc]Tc-labeled cyc-DX600-HYNIC as a SPECT probe for ACE2-specific pancreatic cancer imaging.
Am J Nucl Med Mol Imaging
April 2024
Shanghai Institute of Applied Physics, Chinese Academy of Sciences Shanghai 201800, China.
As a regulator in renin-angiotensin-aldosterone system, angiotensin-converting enzyme 2 (ACE2) closely correlated with tumor progression of pancreatic cancer, meantime, was easily affected by a variety of factors. [Tc]Tc-cyc-DX600 SPECT was established as an ACE2-specific imaging protocol to figure out the ACE2 status in pancreatic tumor. BALB/C-NU mice were used to prepare the subcutaneous cell derived xenograft (CDX) models with HEK-293T or HEK-293T/hACE2 cells to validate ACE2 specificity of [Tc]Tc-cyc-DX600 SPECT and establish SPECT imaging protocol.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!