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Microsatellite loci in the tiger shark and cross-species amplification using pyrosequencing technology. | LitMetric

AI Article Synopsis

  • * Researchers used next generation sequencing to identify nine microsatellite loci from 29 tiger shark samples collected in the western Atlantic, revealing a total of 48 genetic variants (alleles).
  • * The study suggests that these identified genetic markers could help with the conservation and population assessment of tiger sharks and potentially other shark species globally.

Article Abstract

The tiger shark (Galeocerdo cuvier) has a global distribution in tropical and warm temperate seas, and it is caught in numerous fisheries worldwide, mainly as bycatch. It is currently assessed as near threatened by the International Union for Conservation of Nature (IUCN) Red List. In this study, we identified nine microsatellite loci through next generation sequencing (454 pyrosequencing) using 29 samples from the western Atlantic. The genetic diversity of these loci were assessed and revealed a total of 48 alleles ranging from 3 to 7 alleles per locus (average of 5.3 alleles). Cross-species amplification was successful at most loci for other species such as Carcharhinus longimanus, C. acronotus and Alopias superciliosus. Given the potential applicability of genetic markers for biological conservation, these data may contribute to the population assessment of this and other species of sharks worldwide.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5012283PMC
http://dx.doi.org/10.7717/peerj.2205DOI Listing

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