AI Article Synopsis
- Many patients globally experience cardiovascular issues linked to thrombosis, highlighting the need for an effective molecular imaging technique for early detection.
- Fibrin, a key component of blood clots and absent in circulation, serves as a specific target for imaging thrombi, and the study compares linear and cyclic fibrin-binding peptides for their effectiveness.
- Findings show that the cyclic peptide has high stability in human plasma, fast blood clearance, and allows for the imaging of thrombosis in live rabbit models, making it a promising candidate for improving molecular imaging in clinical settings.
Article Abstract
Purpose: Many patients worldwide suffer from cardiovascular diseases for which an underlying factor is thrombosis. Devising a molecular imaging technique for early detection of thrombosis in a clinical setting is highly recommended. Because fibrin is a major constituent of clots and is present in all types of thrombi but absent in circulation, it is a highly specific and sensitive target for molecular imaging of thrombi. It is assumed that cyclization of peptides will improve the receptor binding affinity and stability of the peptide. In the present study, we have developed linear and cyclic fibrin-binding peptides for thrombus imaging and compared their biological properties.
Procedures: Linear HYNIC-GPRPP and cyclic HYNIC-CGPRPPC peptides were synthesized using a standard Fmoc strategy and radiolabeled with Tc-99m. The stability of the radiolabeled peptides in human plasma and their affinity for fibrin and blood clots were determined. Blood clearance and biodistribution were evaluated in rats and mice, respectively. The peptide with the highest affinity was injected to a live rabbit femoral thrombosis model, and scintigraphic images were obtained.
Results: In vitro studies show that peptides are stable in human plasma and have a high affinity for human fibrin. They also demonstrated fast blood clearance in rats and high thrombus uptake in the Balb/c mice femoral thrombosis model. Femoral thrombosis was visualized 30 min postinjection of cyclic peptide in a live rabbit model using single photon emission computed tomography (SPECT)/X-ray computed tomography.
Conclusions: The results indicate that the cyclic peptide is a promising agent for molecular imaging of fibrin using SPECT.
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| http://dx.doi.org/10.1007/s11307-016-1004-3 | DOI Listing |
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