AI Article Synopsis
- Three water-soluble tetracationic quadrupolar chromophores were developed for use in biological imaging, featuring boron π-acceptor groups and thiophene bridges.
- Compound 3 demonstrated long-term stability, high fluorescence efficiency, and strong two-photon absorption, making it a standout with a TPA cross-section of 268 GM at 800 nm in water.
- Live cell studies showed that compound 3 localized in mitochondria and was biocompatible, highlighting its potential for advanced fluorescence imaging of mitochondrial activity in cells.
Article Abstract
Three water-soluble tetracationic quadrupolar chromophores comprising two three-coordinate boron π-acceptor groups bridged by thiophene-containing moieties were synthesised for biological imaging applications. Compound 3 containing the bulkier 5-(3,5-Me2 C6 H2 )-2,2'-(C4 H2 S)2 -5'-(3,5-Me2 C6 H2 ) bridge is stable over a long period of time, exhibits a high fluorescence quantum yield and strong one- and two-photon absorption (TPA), and has a TPA cross section of 268 GM at 800 nm in water. Confocal laser scanning fluorescence microscopy studies in live cells indicated localisation of the chromophore at the mitochondria; moreover, cytotoxicity measurements proved biocompatibility. Thus, chromophore 3 has excellent potential for one- and two-photon-excited fluorescence imaging of mitochondrial function in cells.
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| http://dx.doi.org/10.1002/chem.201602639 | DOI Listing |
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