AI Article Synopsis
- A new outbreak of pseudorabies virus (PRV) variant strains is occurring in China, specifically of genotype II, and the existing Bartha-K61 vaccine is ineffective against these variants.
- * Researchers developed a method using CRISPR-Cas9 technology to rapidly edit the PRV genome, creating a specific genetically modified strain (HeN1) that shows full attenuation in mice.
- * This modified strain not only was safe but also provided immune protection against future PRV infections, highlighting the potential of CRISPR-Cas9 for effective PRV control as new variants arise.
Article Abstract
Currently, pseudorabies virus (PRV) variant strains are outbreaking in China; these variants belong to genotype II PRV. The traditional Bartha-K61 vaccine has failed to provide complete protection against the emergent variant strains. Therefore, rapid attenuation of current epidemic strains is needed for effective PRV control. In this study, we report a rapid method for editing the PRV genome using the CRISPR-Cas9 system. We developed a triple gE/gI/TK gene-inactivated HeN1 PRV strain, because mice were more susceptible to PRV infection, we then evaluated the attenuation of PRV in the mice and demonstrated that modified PRV was fully attenuated. Furthermore, the attenuated strain also induced immune protection in response to a parental PRV challenge. Overall, we showed that PRVs can be rapidly attenuated using CRISPR-Cas9 technology, which will be critical for PRV control, especially when new variant PRV strains emerge.
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| http://dx.doi.org/10.1016/j.virusres.2016.09.004 | DOI Listing |
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