α-Amylase from Anoxybacillus sp. SK3-4 (ASKA) is a thermostable enzyme that produces a high level of maltose from starches. A truncated ASKA (TASKA) variant with improved expression and purification efficiency was characterized in an earlier study. In this work, TASKA was purified and immobilized through covalent attachment on three epoxide (ReliZyme EP403/M, Immobead IB-150P, and Immobead IB-150A) and an amino-epoxide (ReliZyme HFA403/M) activated supports. Several parameters affecting immobilization were analyzed, including the pH, temperature, and quantity (mg) of enzyme added per gram of support. The influence of the carrier surface properties, pore sizes, and lengths of spacer arms (functional groups) on biocatalyst performances were studied. Free and immobilized TASKAs were stable at pH 6.0-9.0 and active at pH 8.0. The enzyme showed optimal activity and considerable stability at 60 °C. Immobilized TASKA retained 50% of its initial activity after 5-12 cycles of reuse. Upon degradation of starches and amylose, only immobilized TASKA on ReliZyme HFA403/M has comparable hydrolytic ability with the free enzyme. To the best of our knowledge, this is the first report of an immobilization study of an α-amylase from Anoxybacillus spp. and the first report of α-amylase immobilization using ReliZyme and Immobeads as supports.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273902 | PMC |
| http://dx.doi.org/10.3390/molecules21091196 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characterization and functional insights of the novel RC-type plasmid pAnox1 from Anoxybacillus gonensis 05S15.
Plasmid
December 2024
Karadeniz Technical University, Faculty of Science, Department of Molecular Biology and Genetics, 61080 Trabzon, Turkey. Electronic address:
The plasmid pAnox1, isolated from Anoxybacillus gonensis 05S15, was sequenced and characterized as a circular, double-stranded DNA molecule of 1592 base pairs with a GC content of 40.01 %. Despite its cryptic nature and small genome, bioinformatic analyses identified conserved motifs associated with replication-related proteins, though BLAST searches revealed no significant homology with other plasmids.
View Article and Find Full Text PDFSalivary microbiota composition before and after use of proton pump inhibitors in patients with laryngopharyngeal reflux: a self-control study.
BMC Oral Health
October 2024
Senior Department of Otolaryngology Head and Neck Surgery, the Sixth Medical Center, Chinese PLA General Hospital, Chinese PLA Medical School, Beijing, 100048, China.
Article Synopsis
- This study investigates how proton pump inhibitors (PPIs) impact the saliva microbiota of laryngopharyngeal reflux (LPR) patients by analyzing saliva samples before and after an 8-week PPI regimen.
- Results showed a significant change in both the diversity and composition of saliva microbiota post-PPI usage, highlighting a shift in bacterial populations.
- Key findings indicate an increase in certain harmful bacteria and a decrease in beneficial bacteria, alongside changes in metabolic gene functions in the saliva microbiota after PPI treatment.
The impact of carvacrol on the larval gut bacterial structure and function of .
Front Microbiol
September 2024
College of Forestry, Northeast Forestry University, Harbin, China.
Introduction: The gut bacteria of insects play an important role in regulating their metabolism, immune system and metabolizing pesticides. Our previous results indicate that carvacrol has certain gastric toxic activity on larvae and affects their detoxification metabolism at the mRNA level. However, the impact of carvacrol on the gut bacteria of larvae has been unclear.
View Article and Find Full Text PDFBacterial microbiome diversity along poultry slaughtering lines: insights from chicken carcasses and environmental sources.
J Vet Res
September 2024
Department of Network Technologies, Turkish Academic Network and Information Center, Scientific and Technological Research Council of Turkey (TÜBiTAK ULAKBİM), 06800 Ankara, Turkey.
Article Synopsis
- The study looked at the variety of bacteria on chicken carcasses and in the area around them during different stages of the slaughter process.
- Researchers collected samples from different points at a chicken processing plant in Turkey over a few months to see how bacterial populations changed.
- They found that bacteria increased after chilling and storage, and that the most bacteria were on the chicken after it was stored, which can help identify where contamination might happen during slaughtering.
Phylogenomic and molecular marker based studies to clarify the evolutionary relationships amongst species and demarcation of the family and some of its constituent genera.
Int J Syst Evol Microbiol
September 2024
Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario CA L8N 3Z5, Canada.
The family was recently proposed encompassing the genera , , , and . Of these genera, contains >50% of the species. However, species form multiple unrelated clades in phylogenetic trees and their evolutionary relationships are unclear.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!