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Fast Neuronal Calcium Signals in Brain Slices Loaded With Fluo-4 AM Ester.
Eur J Neurosci
January 2025
Université Grenoble Alpes, CNRS, LIPhy, Grenoble, France.
Staining brain slices with acetoxymethyl ester (AM) Ca dyes is a straightforward procedure to load multiple cells, and Fluo-4 is a commonly used high-affinity indicator due to its very large dynamic range. It has been shown that this dye preferentially stains glial cells, providing slow and large Ca transients, but it is questionable whether and at which temporal resolution it can also report Ca transients from neuronal cells. Here, by electrically stimulating mouse hippocampal slices, we resolved fast neuronal signals corresponding to 1%-3% maximal fluorescence changes.
View Article and Find Full Text PDFSeizures Triggered by Systemic Administration of 4-Aminopyridine in Rats Lead to Acute Brain Glucose Hypometabolism, as Assessed by [F]FDG PET Neuroimaging.
Int J Mol Sci
November 2024
Unidad de Cartografía Cerebral, Instituto Pluridisciplinar, Universidad Complutense de Madrid, 28040 Madrid, Spain.
4-aminopyridine (4-AP) is a non-selective blocker of voltage-dependent K channels used to improve walking in multiple sclerosis patients, and it may be useful in the treatment of cerebellar diseases. In animal models, 4-AP is used as a convulsant agent. When administered intrahippocampally, 4-AP induces acute local glucose hypermetabolism and significant brain damage, while i.
View Article and Find Full Text PDFBinding of -[Ru(phen)(3,4Apy)] to Model Lipid Membranes: Implications for New Tools in the Development of Antiamyloid Drugs.
Langmuir
December 2024
Department of Chemistry, Federal University of São Carlos, CP 676, CEP, São Carlos, São Paulo 13565-905, Brazil.
This study explores the interactions of the -[Ru(phen)(Apy)] complex (RuApy, phen = 1,10-phenanthroline, Apy = 3,4-aminopyridine) with model lipid membranes to explain the role this complex plays in mitigating Aβ toxicity in PC12 neuronal cells. Fluorescence quenching, surface pressure isotherms in Langmuir monolayers, and infrared reflection-absorption analyses revealed that the positively charged RuApy interacts with the phosphate headgroups of monolayers, indirectly affecting ester carbonyl groups through hydrogen bonding with the amino group of the pyridine ligand of RuApy. These results offer a scenario for the protective effect of RuApy against Aβ toxicity in neuronal cells in which these interactions shield the electrostatic interactions of Aβ with lipid membranes, preserving membrane integrity and mitigating the deleterious influence of Aβ.
View Article and Find Full Text PDFPotassium channels mediate nitric oxide-induced vasorelaxation in arteries supplying colon cancer.
Prostaglandins Other Lipid Mediat
December 2024
Department of Biology, College of Science, University of Zakho, Duhok, Kurdistan Region, Iraq; Department of Biology, College of Science, University of Nawroz, Duhok, Kurdistan Region, Iraq.
Introduction: Aberrant vascular function and cancer growth are closely related, with nitric oxide (NO) being a key factor in vascular tone regulation. This study provides Novel insights into the distinctive mechanisms underlying cancer-associated vascular dysfunction by investigating the involvement of potassium (K) channels in NO-mediated vasorelaxation within arteries supplying colon cancer.
Methods: Arterial segments from colon cancer patients were isolated and sectioned into rings, these rings were mounted in an organ bath filled with Krebs' solution and maintained at 37°C.
Investigation of the Effects of Blocking Potassium Channels With 4-Aminopyridine on Paclitaxel Activity in Breast Cancer Cell Lines.
Cancer Rep (Hoboken)
December 2024
School of Medicine, Biophysics Department, T.C. Marmara University, Maltepe, İstanbul, Turkey.
Background: Paclitaxel (PTX) has been used as a chemotherapeutic agent for several malignancies, including breast cancer, and efforts to increase the efficiency of PTX are continuous. Previous studies have shown that the voltage-gated K channels are over-expressed in breast cancer cell lines; therefore, blocking this type of K channel reduces cell proliferation and viability.
Aims: In this study, FDA-approved 4-aminopyridine (4-AP), a voltage-gated potassium channel blocker, was used in combination with PTX to improve the anticancer activity of PTX in MCF-7 and MDA-MB-231 cell lines.
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