Toxoplasmosis, a zoonotic parasitic disease, is a huge challenge for which there is no effective vaccine up till now. In this study, chitosan nanospheres encapsulated with Toxoplasma lysate vaccine was evaluated for its ability to protect mice against both acute and chronic toxoplasmosis models of infection. Results showed that chitosan nanospheres were equally effective to Freund's incomplete adjuvant (FIA) in enhancing the efficacy of Toxoplasma lysate vaccine. The effectiveness was demonstrated by the delayed death of vaccinated mice following challenge either with virulent RH or avirulent Me49 strains, the significant decrease in parasite density in different organs, significant increase in the humoral and cellular immune response (IgG and IFN γ) with a marked reduction of pathological changes in the different organs. However chitosan nanospheres were superior to FIA due to their cost effective preparation and much less necrotic changes induced in the studied organs. The success of chitosan polymer as an alternative to commonly used adjuvants paves the way for the use of other newly developed polymers to be used in the field of vaccine development.
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| http://dx.doi.org/10.1007/s12639-014-0546-z | DOI Listing |
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Article Synopsis
- The study investigates the use of poly-lactic-co-glycolic acid (PLGA) and chitosan (CS) as delivery systems for a ribosome protein (TgRPS2) to create potential vaccines against a harmful zoonotic disease affecting humans and animals.
- Researchers synthesized TgRPS2-PLGA and TgRPS2-CS nanospheres using novel techniques and evaluated their efficacy and toxicity before immunizing mice to assess immune responses.
- Results indicated that the nanospheres effectively stimulated immune responses, increased specific immune cell populations, and inhibited pathogen replication in affected tissues, suggesting their potential as preventive agents against toxoplasmosis.
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