AI Article Synopsis
- Galactomannan is a key component of the cell wall in Aspergillus fumigatus, consisting of a core mannan structure with mannose and galactofuran side chains.
- Researchers deleted 11 genes related to mannosyltransferases involved in creating mannose linkages to study mannan biosynthesis, but found no significant impact on mannan levels in the mycelium.
- However, these gene deletions did decrease mannan content in the conidial cell walls, causing structural disorganization and reduced survival of conidia both in lab settings and in living organisms.
Article Abstract
The galactomannan is a major cell wall molecule of Aspergillus fumigatus. This molecule is composed of a linear mannan with a repeating unit composed of four α1,6 and α1,2 linked mannose with side chains of galactofuran. To obtain a better understanding of the mannan biosynthesis in A. fumigatus, it was decided to undertake the successive deletion of the 11 genes which are putative orthologs of the mannosyltransferases responsible for establishing α1,6 and α1,2 mannose linkages in yeast. These deletions did not lead to a reduction of the mannan content of the cell wall of the mycelium of A. fumigatus. In contrast, the mannan content of the conidial cell wall was reduced and this reduction was associated with a partial disorganization of the cell wall leading to defects in conidial survival both in vitro and in vivo.
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| http://dx.doi.org/10.1111/cmi.12665 | DOI Listing |
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