AI Article Synopsis
- - A method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed to detect irradiation histories in raw beef livers by measuring the compound 5,6-dihydrothymidine (DHdThd).
- - The process involved extracting liver DNA, digesting it enzymatically, and purifying nucleosides, followed by analysis where DHdThd levels increased in a dose-dependent manner after γ-irradiation between 1.0-11.3 kGy.
- - This technique shows promise for identifying irradiation history in food products where DNA can be extracted, particularly noting consistent dose-response results across different beef livers.
Article Abstract
A method for detecting irradiation histories of raw beef livers was developed by measuring 5,6-dihydrothymidine (DHdThd) using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Liver DNA was extracted using phenol-chloroform extraction followed by precipitation in 50% ethanol. DNA was then enzymatically digested and nucleosides were purified using an OASIS MCX column. DHdThd and thymidine (dThd) contents of resulting test solutions were analyzed using LC-MS/MS. DHdThd was detected specifically after γ-irradiation. Concentration ratios of DHdThd to dThd in the test solutions increased dose-dependently after irradiation at 1.0-11.3kGy, which included the practical dose for sterilization of 2-7kGy. Dose-response curves from beef livers of individual animals almost overlapped. Thus, this method is a candidate for the detection of irradiation histories of foods from which DNA can be extracted.
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| http://dx.doi.org/10.1016/j.foodchem.2016.08.036 | DOI Listing |
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