Promotion of cells to close gaps and encourage cell coverage, by radio frequency glow discharge treatment.

Exposure of dental abutments to cleaning and sterilizing Radio Frequency Glow Discharge Treatment (RFGDT) triggered greater degrees of human gingival fibroblast (HGF) attachment and spreading over their surfaces. Enhanced cell growth and metabolic activity of such HGFs were found which might lead to improved cellular margins in the smile-revealing "esthetic zone". This investigation, approved by the Institutional Review Board, employed in vitro studies of HGFs to support in vivo clinical applications of differentially treated titanium healing abutments to demonstrate the possible improvements for tissue growth around dental implants. Harvested commercially pure titanium (cpTi) abutments from three clinical cases per group revealed that separation of the abutments from the human gingival tissues occurred mainly intercellularly rather than directly from the tissue, suggesting that placement of an RFGDT permanent abutment would trigger tissue-integration more completely than noted with usual alcohol-cleaned abutments. This work confirmed and extended observations of prior studies that RFGDT materials have mitogenic effects that might be captured for stimulating desired tissue growth around implanted biomaterial appliances. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 169-177, 2017.