AI Article Synopsis
- The study focused on how liposomal nanocontainers linked to monoclonal antibodies for VEGF, VEGFR2, and other tumor proteins were absorbed by poorly differentiated tumor cells in lab cultures.
- The researchers used laser scanning confocal microscopy to compare how well these antibody-linked liposomes and regular liposomes entered and accumulated in the tumor cells.
- Results showed that the antibody-linked liposomes penetrated and accumulated in the tumor cells nearly twice as effectively as the non-linked ones, and their entry relied on a specific clathrin-dependent endocytosis process.
Article Abstract
Internalization of liposomal nanocontainers conjugated with monoclonal antibodies to VEGF, VEGFR2 (KDR), and proteins overproduced in the tumor tissue was studied in vitro on cultures of poorly differentiated tumor cells. Comparative analysis of accumulation of vectored liposomes in the tumor cells was performed by evaluating co-localization of labeled containers and cell organelles by laser scanning confocal microscopy. We observed nearly 2 times more active penetration and accumulation of liposomes vectored with antibodies in the tumor cells in comparison with non-vectored liposomes. Selective clathrin-dependent penetration of vectored liposomes into tumor cells was demonstrated by using pharmacological agents inhibiting endocytosis.
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| http://dx.doi.org/10.1007/s10517-016-3466-3 | DOI Listing |
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