AI Article Synopsis
- Human sera are typically used as controls for diagnostic tests but face issues like limited availability, variable quality, and high costs.
- This study introduces a chimeric molecule (CD64-IgE Fc) that combines human CD64 and IgE Fc domains to create allergen-specific artificial sera.
- The research showed these artificial sera can effectively replace human sera in diagnosing over 20 allergens, achieving comparable results in tests like RAST with a high level of accuracy.
Article Abstract
Human sera are the first choice as controls for diagnostic applications such as immunoassays, but are limited regarding availability, varying quality, and high costs. In this study, we aimed to circumvent these limitations by the use of a chimeric adaptor molecule comprising the extracellular domains of the human FcγRI (CD64) fused with human IgE Fc domains (CD64-IgE Fc). Allergen-specific antibodies were produced in rabbits using eight different allergens, extracts, and allergen mixtures including mites, pollen, drugs, and food. Preincubation of polyclonal IgG with CD64-IgE Fc established allergen-specific artificial sera that showed comparable results for more than 20 allergens and allergen extracts in three diagnostic systems for the determination of specific IgE. The agreement for these artificial sera is within ±1 radioallergosorbent test (RAST) class. Hence, rabbit IgG complexed with the IgG-specific CD64-IgE Fc adaptor molecule could provide a substitute for human reference sera with specificity for virtually any protein of interest.
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| http://dx.doi.org/10.1111/all.13038 | DOI Listing |
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