AI Article Synopsis
- Acetonitrile (ACN) is a solvent that promotes the formation of β-sheet structures in proteins, particularly hemoglobin (Hb).
- Several techniques, including fluorescence experiments and spectroscopy, were used to observe the structural changes in Hb when exposed to different concentrations of ACN.
- At lower ACN concentrations, Hb shows a partially unfolded state, while higher concentrations lead to the aggregation of Hb, confirmed by imaging techniques.
Article Abstract
Acetonitrile is a mild solvent, which induces β-sheet conformation in proteins. The global conformational changes in Hb in the presence of ACN were studied using intrinsic fluorescence experiments, acrylamide quenching, ANS fluorescence measurements, soret absorbance spectroscopy, fourier transform infrared spectroscopy, circular dichroism, thioflavin T and congo red assay. Molecular docking showed the binding of hydrophobic residues of Hb to ACN. Hb exists as a partially unfolded intermediate state at 30% v/v ACN. Hb aggregates were obtained at 60% v/v ACN concentration, which were further confirmed by transmission electron microscopy.
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| http://dx.doi.org/10.2174/0929866523666160831154706 | DOI Listing |
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