AI Article Synopsis
- - Atherosclerosis is a major contributor to cardiovascular diseases, which are the top cause of death globally, and the role of long noncoding RNAs (lncRNAs) in this process is not fully understood.
- - This study identified that lncRNA HOXC-AS1 and HOXC6 are reduced in atherosclerotic conditions and confirmed these findings using various molecular analysis techniques.
- - Overexpressing HOXC-AS1 in macrophages increased HOXC6 levels, and it was found that oxidized low-density lipoprotein (Ox-LDL) lowered both HOXC-AS1 and HOXC6 expression; however, boosting HOXC-AS1 helped reduce cholesterol buildup caused by Ox-LDL.
Article Abstract
Atherosclerosis is a common pathological basis of cardiovascular disease, which remains the leading cause of mortality. Long noncoding RNAs (lncRNAs) are newly studied non-protein-coding RNAs involved in gene regulation, but how lncRNAs exert regulatory effect on atherosclerosis remains unclear. In this study, we found that lncRNA HOXC cluster antisense RNA 1 (HOXC-AS1) and homeobox C6 (HOXC6) were downregulated in carotid atherosclerosis by performing microarray analysis. The results were verified in atherosclerotic plaques and normal arterial intima tissues by quantitative reverse transcription PCR and western blot analysis. Lentivirus-mediated overexpression of HOXC-AS1 induced HOXC6 expression at mRNA and protein levels in THP-1 macrophages. Besides, oxidized low-density lipoprotein (Ox-LDL) decreased expression of HOXC-AS1 and HOXC6 in a time-dependent manner. Induction of cholesterol accumulation by Ox-LDL could be partly suppressed by overexpression of HOXC-AS1.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1089/dna.2016.3422 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!