We show how a pyramid phase microscope can be used to obtain tomographic information of the spatial variation of refractive index in biological samples using the Radon transform. A method that uses the information provided by the phase microscope for axial and lateral repositioning of the sample when it rotates is also described. Its application to the reconstruction of mouse embryos in the blastocyst stage is demonstrated.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4986812 | PMC |
| http://dx.doi.org/10.1364/BOE.7.003049 | DOI Listing |
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