AI Article Synopsis
- The study investigates how human gastric cancer mesenchymal stem cells (hGC-MSCs) affect tumor growth and progression in gastric cancer-bearing mice.
- Mice with gastric cancer were injected with different amounts of hGC-MSCs and compared to a control group receiving PBS; tumor volume and molecular changes were measured.
- Results showed that hGC-MSCs significantly increased tumor size and altered the expression levels of key molecules associated with proliferation and invasion, suggesting they enhance cancer growth through these mechanisms.
Article Abstract
Objective: To study the effect of hGC-MSCs from human gastric cancer tissue on cell proliferation, invasion and epithelial-mesenchymal transition in tumor tissue of gastric cancer tumor-bearing mice.
Methods: BABL/c nude mice were selected as experimental animals and gastric cancer tumor-bearing mice model were established by subcutaneous injection of gastric cancer cells, randomly divided into different intervention groups. hGC-MSCs group were given different amounts of gastric cancer cells for subcutaneous injection, PBS group was given equal volume of PBS for subcutaneous injection. Then tumor tissue volume were determined, tumor-bearing mice were killed and tumor tissues were collected, mRNA expression of proliferation, invasion, EMT-related molecules were determined.
Results: 4, 8, 12, 16, 20 d after intervention, tumor tissue volume of hGC-MSCs group were significantly higher than those of PBS group and the more the number of hGC-MSCs, the higher the tumor tissue volume; mRNA contents of Ki-67, PCNA, Bcl-2, MMP-2, MMP-7, MMP-9, MMP-14, N-cadherin, vimentin, Snail and Twist in tumor tissue of hGC-MSCs group were higher than those of PBS group, and mRNA contents of Bax, TIMP1, TIMP2 and E-cadherin were lower than those of PBS group.
Conclusion: hGC-MSCs from human gastric cancer tissue can promote the tumor growth in gastric cancer tumor-bearing mice, and the molecular mechanism includes promoting cell proliferation, invasion and epithelial-mesenchymal transition.
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| http://dx.doi.org/10.1016/j.apjtm.2016.06.004 | DOI Listing |
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