AI Article Synopsis
- The Yersinia entomophaga toxin complex (Yen-TC) is a key factor in the bacterium's ability to cause disease in insects.
- A method has been developed for isolating and purifying Yen-TC efficiently using size exclusion chromatography from a bacterial culture.
- This purification process can yield about 2 mg of Yen-TC with a high purity level of 95-98% from a 100-ml culture overnight.
Article Abstract
The Yersinia entomophaga toxin complex (Yen-TC) is the bacterium's main virulence determinant. Because of its high insect activity, methods were developed to allow the routine isolation and purification of Yen-TC from an overnight bacterial culture using size exclusion chromatography. Here we outline an overnight purification procedure using a 100-ml culture volume, where approximately 2 mg of Yen-TC, with an approximate purity of 95-98 %, can be routinely obtained.
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| http://dx.doi.org/10.1007/978-1-4939-6367-6_4 | DOI Listing |
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