International journal of systematic and evolutionary microbiology

1466-503466112016NovInternational journal of systematic and evolutionary microbiologyInt J Syst Evol MicrobiolBorrelia mayonii sp. nov., a member of the Borrelia burgdorferi sensu lato complex, detected in patients and ticks in the upper midwestern United States.487848804878-488010.1099/ijsem.0.001445Lyme borreliosis (LB) is a multisystem disease caused by spirochetes in the Borrelia burgdorferisensu lato (Bbsl) genospecies complex. We previously described a novel Bbsl genospecies (type strain MN14-1420T) that causes LB among patients with exposures to ticks in the upper midwestern USA. Patients infected with the novel Bbsl genospecies demonstrated higher levels of spirochetemia and somewhat differing clinical symptoms as compared with those infected with other Bbsl genospecies. The organism was detected from human specimens using PCR, microscopy, serology and culture. The taxonomic status was determined using an eight-housekeeping-gene (uvrA, rplB, recG, pyrG, pepX, clpX, clpA and nifS) multi-locus sequence analysis (MLSA) and comparison of 16S rRNA gene, flaB, rrf-rrl, ospC and oppA2 nucleotide sequences. Using a system threshold of 98.3 % similarity for delineation of Bbsl genospecies by MLSA, we demonstrated that the novel species is a member of the Bbsl genospecies complex, most closely related to B. burgdorferisensu stricto (94.7-94.9 % similarity). This same species was identified in Ixodes scapularis ticks collected in Minnesota and Wisconsin. This novel species, Borrelia mayonii sp. nov, is formally described here. The type strain, MN14-1420, is available through the Deutsche Sammlung von Mikroorganismen und Zelkulturen GmbH (DSM 102811) and the American Type Culture Collection (ATCC BAA-2743).PrittBobbi SBSDepartment of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.Respicio-KingryLaurel BLBDivision of Vector Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA.SloanLynne MLMDepartment of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.SchrieferMartin EMEDivision of Vector Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA.ReplogleAdam JAJDivision of Vector Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA.BjorkJennaJMinnesota Department of Health, St. Paul, MN, USA.LiuGongpingGMinnesota Department of Health, St. Paul, MN, USA.KingryLuke CLCDivision of Vector Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA.MeadPaul SPSDivision of Vector Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA.NeitzelDavid FDFMinnesota Department of Health, St. Paul, MN, USA.SchiffmanElizabethEMinnesota Department of Health, St. Paul, MN, USA.Hoang JohnsonDiep KDKWisconsin Department of Health Services, Madison, WI, USA.DavisJeffrey PJPWisconsin Department of Health Services, Madison, WI, USA.PaskewitzSusan MSMUniversity of Wisconsin-Madison, Madison, WI, USA.BoxrudDavidDMinnesota Department of Health, St. Paul, MN, USA.DeedonAleciaAWisconsin Department of Health Services, Madison, WI, USA.LeeXiaXUniversity of Wisconsin-Madison, Madison, WI, USA.MillerTracy KTKNorth Dakota Department of Health, Bismarck, ND, USA.FeistMichelle AMANorth Dakota Department of Health, Bismarck, ND, USA.StewardChristopher RCRWisconsin Department of Health Services, Madison, WI, USA.TheelElitza SESDepartment of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.PatelRobinRDepartment of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.IrishCole LCLDepartment of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.PetersenJeannine MJMDivision of Vector Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA.engCC999999Intramural CDC HHSUnited StatesJournal Article20160824