AI Article Synopsis
- - A new human embryonic stem cell line with a MIXL1-eGFP reporter was created using TALEN genome engineering, allowing for tracking of MIXL1 expression.
- - This reporter effectively identifies mesendodermal precursor cells, providing insight into specific cell lineage.
- - The MIXL1-eGFP reporter line is valuable for researching and isolating MIXL1 cell populations, enhancing our understanding of their development and mechanisms.
Article Abstract
We have generated a MIXL1-eGFP reporter human embryonic stem cell (hESC) line using TALEN-based genome engineering. This line accurately traces endogenous MIXL1 expression via an eGFP reporter to mesendodermal precursor cells. The utility of the MIXL1-eGFP reporter hESC line lies in the prospective isolation, lineage tracing, and developmental and mechanistic studies of MIXL1 cell populations.
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| http://dx.doi.org/10.1016/j.scr.2016.05.010 | DOI Listing |
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