It is essential that any DNA barcode reference library be based upon correctly identified specimens. The Barcode of Life Data Systems (BOLD) requires information such as images, geo-referencing, and details on the museum holding the voucher specimen for each barcode record to aid recognition of potential misidentifications. Nevertheless, there are misidentifications and incomplete identifications (e.g., to a genus or family) on BOLD, mainly for species from tropical regions. Unfortunately, experts are often unavailable to correct taxonomic assignments due to time constraints and the lack of specialists for many groups and regions. However, considerable progress could be made if barcode records were available for all type specimens. As a result of recent improvements in analytical protocols, it is now possible to recover barcode sequences from museum specimens that date to the start of taxonomic work in the 18th century. The present study discusses success in the recovery of DNA barcode sequences from 2805 type specimens of geometrid moths which represent 1965 species, corresponding to about 9% of the 23 000 described species in this family worldwide and including 1875 taxa represented by name-bearing types. Sequencing success was high (73% of specimens), even for specimens that were more than a century old. Several case studies are discussed to show the efficiency, reliability, and sustainability of this approach.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1139/gen-2015-0197 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Diffusion-weighted MRI of advanced gastric cancer: correlations of the apparent diffusion coefficient with Borrmann classification, proliferation and aggressiveness.
Abdom Radiol (NY)
January 2025
Department of Medical Imaging, Tongji Hospital, Tongji University, No. 389, Xincun Road, Putuo District, Shanghai, 200065, China.
Objectives: This study aimed to compare apparent diffusion coefficient (ADC) values derived from diffusion-weighted imaging (DWI) of different Borrmann types of advanced gastric cancer (AGC) and correlate these ADC values with Ki-67 expression and serum CEA levels in AGC.
Methods: A total of 84 patients with AGC who underwent DWI of the upper abdomen before tumor resection in our hospital between June 2014 and July 2018 were included in the present study. DWI was obtained with a single-shot echo planar imaging sequence in the axial plane (b values: 0, 100, 700 and 1000 s/mm).
Exploring the role of volume energy density in altering microstructure and corrosion behavior of nitinol alloys produced by laser powder bed fusion.
Sci Rep
January 2025
I-Form Advanced Manufacturing Research Centre, Dublin City University, Dublin, Ireland.
In the realm of materials science and engineering, the pursuit of advanced materials with tailored properties has been a driving goal behind technological progress. Scientific interest in laser powder bed fusion (L-PBF) fabricated NiTi alloy has in recent times seen an upsurge of activity. In this study, we investigate the impact of varying volume energy density (VED) during L-PBF on the microstructure and corrosion behaviour of NiTi alloys in both scan (XY) and built (XZ) planes.
View Article and Find Full Text PDFObjective surgical skill assessment based on automatic recognition of dissection and exposure times in robot-assisted radical prostatectomy.
Langenbecks Arch Surg
January 2025
Department for the Promotion of Medical Device Innovation, National Cancer Center Hospital East, 6-5-1, Kashiwanoha, Kashiwa, Chiba, 277-8577, Japan.
Purpose: Assessing surgical skills is vital for training surgeons, but creating objective, automated evaluation systems is challenging, especially in robotic surgery. Surgical procedures generally involve dissection and exposure (D/E), and their duration and proportion can be used for skill assessment. This study aimed to develop an AI model to acquire D/E parameters in robot-assisted radical prostatectomy (RARP) and verify if these parameters could distinguish between novice and expert surgeons.
View Article and Find Full Text PDFA non-randomised open-label exploratory 'window of opportunity' study of TG02 treatment in patients with locally advanced primary and recurrent mutant colorectal cancer.
Heliyon
January 2025
Sir Peter MacCallum Department of Oncology, The University of Melbourne, Australia.
Background: TG02 is a peptide-based cancer vaccine eliciting immune responses to oncogenic codon 12/13 mutations. This phase 1 clinical trial (NCT02933944) assessed the safety and immunological efficacy of TG02 adjuvanted by GM-CSF in patients with -mutant colorectal cancer.
Methods: In the interval between completing CRT and pelvic exenteration, patients with resectable mutation-positive, locally advanced primary or current colorectal cancer, received 5-6 doses of TG02/GM-CSF.
The smallest plant from the Lower Devonian of South China and the divergent life-history strategies in zosterophyllopsids.
Proc Biol Sci
January 2025
The Key Laboratory of Orogenic Belts and Crustal Evolution, School of Earth and Space Sciences, Peking University, Beijing 100871, People's Republic of China.
Plants have evolved different life-history strategies to overcome limited amounts of available resources; however, when and how divergent strategies of sexual reproduction evolved in early land plants are not well understood. As one of the notable and vital components of early terrestrial vegetation, the Zosterophyllopsida and its type genus reached maximum species diversity during the Pragian (Early Devonian; 410.8-407.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!