Cell Fractionation and Quantitative Analysis of HIV-1 Reverse Transcription in Target Cells.

Bio Protoc

Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, USA.

Published: October 2013

This is a protocol to detect HIV-1 reverse transcription products in cytoplasmic and nuclear fractions of cells infected with VSV-G-pseudotyped envelope-defective HIV-1. This protocol can also be extended to HIV-1 with regular envelope.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4990143PMC
http://dx.doi.org/10.21769/bioprotoc.949DOI Listing

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