AI Article Synopsis
- The study focuses on comparing two nucleic acid (NA) extraction methods: a low-cost manual method (Qiagen kit or salting-out) and an automated, high-cost method (MagNAPure Compact) for molecular diagnosis in microbiology.
- It highlights that while MagNAPure is as effective as the Qiagen method for various clinical specimens, it may not be suitable for extracting NA from blood samples.
- The authors argue that despite its higher cost, the benefits of MagNAPure, such as reduced risk of cross-contamination and less hands-on time, make it a worthwhile option for laboratories.
Article Abstract
The choice of nucleic acids (NAs) extraction method for molecular diagnosis in microbiology is of major importance because of the low microbial load, different nature of microorganisms, and clinical specimens. The NA yield of different extraction methods has been mostly studied using spiked samples. However, information from real human clinical specimens is scarce. The purpose of this study was to compare the performance of a manual low-cost extraction method (Qiagen kit or salting-out extraction method) with the automated high-cost MagNAPure Compact method. According to cycle threshold values for different pathogens, MagNAPure is as efficient as Qiagen for NA extraction from noncomplex clinical specimens (nasopharyngeal swab, skin swab, plasma, respiratory specimens). In contrast, according to cycle threshold values for RNAseP, MagNAPure method may not be an appropriate method for NA extraction from blood. We believe that MagNAPure versatility reduced risk of cross-contamination and reduced hands-on time compensates its high cost.
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| http://dx.doi.org/10.1016/j.diagmicrobio.2016.07.008 | DOI Listing |
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