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Filename: controllers/Detail.php
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Function: _error_handler
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Filename: models/Detail_model.php
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Function: strpos
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Function: insertAPISummary
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Filename: helpers/my_audit_helper.php
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Filename: controllers/Detail.php
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Filename: controllers/Detail.php
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Line: 256
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File: /var/www/html/index.php
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Filename: controllers/Detail.php
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Filename: controllers/Detail.php
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Filename: controllers/Detail.php
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Background: Basophil activation tests (BATs) have promise for research and for clinical monitoring of patients with allergies. However, BAT protocols vary in blood anticoagulant used and temperature and time of storage before testing, complicating comparisons of results from various studies.
Objective: We attempted to establish a BAT protocol that would permit analysis of blood within 24 hours of obtaining the sample.
Methods: Blood from 46 healthy donors and 120 patients with peanut allergy was collected into EDTA or heparin tubes, and samples were stored at 4°C or room temperature for 4 or 24 hours before performing BATs.
Results: Stimulation with anti-IgE or IL-3 resulted in strong upregulation of basophil CD203c in samples collected in EDTA or heparin, stored at 4°C, and analyzed 24 hours after sample collection. However, a CD63 population of basophils was not observed in any conditions in EDTA-treated samples unless exogenous calcium/magnesium was added at the time of anti-IgE stimulation. By contrast, blood samples collected in heparin tubes were adequate for quantification of upregulation of basophil CD203c and identification of a population of CD63 basophils, irrespective of whether the specimens were analyzed by means of conventional flow cytometry or cytometry by time-of-flight mass spectrometry, and such tests could be performed after blood was stored for 24 hours at 4°C.
Conclusion: BATs to measure upregulation of basophil CD203c and induction of a CD63 basophil population can be conducted with blood obtained in heparin tubes and stored at 4°C for 24 hours.
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http://dx.doi.org/10.1016/j.jaci.2016.04.060 | DOI Listing |
J Zoo Wildl Med
December 2024
University of Georgia, College of Veterinary Medicine, Athens, GA 30602, USA.
Anticoagulant choice can have significant effect on complete blood counts of reptiles and has not been reported in bearded dragons (). Whole blood was collected from 14 captive individuals and aliquoted into separate blood tubes containing dipotassium ethylenediaminetetraacetic acid (EDTA) and lithium heparin. Samples were shipped and processed within 24 h.
View Article and Find Full Text PDFPLoS One
December 2024
LADR Laboratory Group Dr. Kramer & Kollegen, Geesthacht, Germany.
Objectives: In most countries the majority of patients are in outpatient care. In difference to hospitalized patients, their blood samples often take hours after collection to centrifugation. The study investigates the release of potassium and the development of pseudohyperkalemia in lithium heparin (Li-Hep) and serum blood collection tubes over time.
View Article and Find Full Text PDFScand J Clin Lab Invest
December 2024
Department of Clinical Biochemistry, Gødstrup Hospital, Herning, Denmark.
Venous blood is considered an acceptable alternative to arterial blood for assessment of metabolic acid-base disorders. Also, venous sampling using lithium-heparin (Li-Hep) tubes is advantageous to arterial sampling using PICO syringes, the risk of complications being lower. Usage of partly filled tubes without firm knowledge about the clinical consequences is, however, a pre-analytic consideration.
View Article and Find Full Text PDFStem Cell Rev Rep
November 2024
INSERM U935/U1310 ESTeam Paris Sud Human Pluripotent Stem Cell Core Facility, Villejuif, France.
The discovery of endothelial progenitor cells has revolutionized our understanding of postnatal blood vessel formation, with endothelial colony-forming cells (ECFCs) emerging as key players in vasculogenesis. Among various ECFC sources, cord blood-derived ECFCs (CB-ECFCs) are of particular interest due to their superior proliferative and clonogenic potential and their ability to promote vascular network formation. Human embryonic stem cell-derived endothelial cells (hESC-ECs) have also shown potential in regenerative medicine, though their vasculogenic efficacy remains unclear compared to CB- and adult blood-derived ECFCs (AB-ECFCs).
View Article and Find Full Text PDFAsian J Neurosurg
December 2024
Department of Neurosurgery, Saiseikai Utsunomiya Hospital, Utsunomiya, Tochigi, Japan.
Hemorrhagic strokes are considered as contraindications of extracorporeal membrane oxygenation (ECMO) therapy because of anticoagulant administration and ECMO-associated coagulopathy. We present a rare case of successful microsurgical clipping under ECMO for a poor-grade subarachnoid hemorrhage (SAH) patient with severe neurogenic pulmonary edema (NPE). A 50-year-old man presenting with the sudden loss of consciousness was diagnosed with poor-grade SAH with severe NPE, and was intubated.
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