The kinetics of isolation of trypsin immobilized on a dialdehyde cellulose carrier in buffer solutions with pH 7.0 and 8.0 was studied. The number of the aldehyde groups amounted to 15-60 per cent of the initial one. The kinetics of hydrolytic destruction of the collected samples with the immobilized trypsin and the carrier in buffer solutions with pH 7.0 and 8.0 was also studied. The constants of the rates of trypsin isolation from the materials and the constants of the rates of liberation into the buffer solutions of the compounds containing CO-groups were determined. The groups were used for estimating hydrolytic destruction of the materials and the time of complete destruction of the materials in the solutions.
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Nucl Med Mol Imaging
February 2025
Nuclear Fuel Research School, Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran.
Purpose: Curcumin as a potent anti-inflammatory and cancer-prevention molecule was labeled with n.c.a Lu.
View Article and Find Full Text PDFSci Rep
January 2025
Department of Archaeology, Faculty of History, Vilnius University, Universiteto St. 7, Vilnius, 01513, Lithuania.
This study explores how major climatic shifts, together with socioeconomic factors over the past two millennia, influenced buffer crop selection, focusing on five crops: rye, millet, buckwheat, oat, and hemp. For this study, we analyzed archaeobotanical data from 135 archaeological contexts and historical data from 242 manor inventories across the northeastern Baltic region, spanning the period from 100 to 1800 AD. Our findings revealed that rye remained a main staple crop throughout the studied periods reflecting environmental adaptation to northern latitudes.
View Article and Find Full Text PDFJ Chromatogr B Analyt Technol Biomed Life Sci
January 2025
Northwest University Chang An Hospital, Faculty of Life Sciences and Medicine, Northwest University, Xi'an, Shaanxi 710069, China; Department of Clinical Pharmaceutics, Chang An District Hospital, Xi'an, Shaanxi 710118, China. Electronic address:
Immobilizing the target protein on a solid surface with controlled orientation, high specificity, and maintained activity is a proven strategy to enhance the stability of the protein. In this study, we employed an ultra-high affinity protein pair consisting of a mutant of colicin E7 Dnase and its corresponding inhibitor, immunity protein 7(Im7), to develop an immobilized α-adrenoceptor (α-AR) column. Briefly, we expressed α-AR fused with CL7 as a tag at its C-terminus in Escherichia coli cells.
View Article and Find Full Text PDFAnal Chem
January 2025
Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitaetsstr. 31, Regensburg 93053, Germany.
To ensure high quality of food and water, the identification of traces of pathogens is mandatory. Rapid nucleic acid-based tests shorten traditional detection times while maintaining low detection limits. Challenging is the loss of nucleic acids during necessary purification processes, since elution off solid surfaces is not efficient.
View Article and Find Full Text PDFJ Integr Plant Biol
January 2025
State Key Laboratory of Rice Biology and Breeding & Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China.
Upon recognizing elicitors derived from herbivores, many plants activate specific defenses. Most of the elicitors identified thus far are from the oral secretions and egg-laying fluids of herbivores; in contrast, herbivore fecal excreta have been sparsely studied in this context. In this study, we identified elicitors in the frass of the striped stem borer (SSB; Chilo suppressalis) larvae using a combination of molecular and chemical analyses, bioactivity tests and insect performance bioassays.
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