Ostrinia furnacalis (Guenée) and Helicoverpa armigera (Hübner) are the most important pests of maize in China. A laboratory study and a 2-year field study on the efficacy of transgenic maize expressing the Cry1Ac protein BT38 against O. furnacalis and H. armigera were performed. We found that the husks, kernels, and silks of BT38 showed significant efficacy against larvae of O. furnacalis and H. armigera. In the field, when neonate larvae of O. furnacalis and H. armigera were on plants at different growth stages and when levels of leaf-damage or number of damaged silks were used to score efficacy, we found that BT38 showed significant insecticidal efficacy against O. furnacalis and H. armigera, but the non-Bt maize did not show significant efficacy against either pest. These results suggest that the insecticidal efficacy of Bt maize expressing the Cry1Ac protein could be useful in the integrated pest management of these key maize pests.
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http://dx.doi.org/10.1007/s11356-016-7340-7 | DOI Listing |
Pest Manag Sci
January 2025
Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
Background: Vegetative insecticidal proteins (Vip3) from Bacillus thuringiensis (Bt) have been utilized for control of lepidopteran insect pests. The majority of known Vip3 proteins possess exceptional high toxicity against Noctuid insects such as the fall armyworm (FAW, Spodoptera frugiperda), beet armyworm (BAW, Spodoptera exigua) and cotton bollworm (CBW, Helicoverpa armigera), but generally have relatively low or even no activity against some very important pest insects, such as Asian corn borer (ACB, Ostrinia furnacalis), European corn borer (ECB, Ostrinia nubilalis), rice stem borer (RSB, Chilo suppressalis) and oriental armyworm (OAW, Mythimna separata).
Results: Here, we report mutant Vip3Af with a single amino acid mutation, Vip3Af-T686R, which gains significantly higher insecticidal activity against ACB, OAW and BAW, while retaining high activity against FAW, CBW and RSB.
Int J Biol Macromol
August 2024
Institute of Plant Protection, Jilin Academy of Agricultural Science/Jilin Key Laboratory of Agricultural Microbiology/Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Gongzhuling 136100, China. Electronic address:
Traditional chemical pesticide dosage forms and crude application methods have resulted in low pesticide utilization, increased environmental pollution, and the development of resistance. Compared to traditional pesticides, nanopesticides enhance the efficiency of pesticide utilization and reduce the quantity required, thereby decreasing environmental pollution. Herein, Cry1Ac insecticidal crystal protein from Bacillus thuringiensis Subsp.
View Article and Find Full Text PDFInsects
December 2023
Department of Grassland Resource and Ecology, College of Grass Science and Technology, China Agricultural University, Beijing 100193, China.
(Hübner) and (Guenée) are the most devastating insect pests at the ear stage of maize, causing significant losses to the sweet corn industry. Pesticide control primarily relies on spraying during the flowering stage, but the effectiveness is inconsistent since larvae are beneath husks within hours to a day, making pesticide treatments simpler to avoid. Insufficient understanding of pest activity patterns impedes precise and efficient pesticide control.
View Article and Find Full Text PDFPlants (Basel)
November 2022
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Lepidopteran pests present a key problem for maize production in China. In order to develop a new strategy for the pest control, the Chinese government has issued safety certificates for insect-resistant transgenic maize, but whether these transformation events can achieve high dose levels to major target pests is still unclear. In this paper, the transformation events of DBN9936 (Bt-Cry1Ab), DBN9936 × DBN9501 (Bt-Cry1Ab + Vip3A), Ruifeng 125 (Bt-Cry1Ab/Cry2Aj), and MIR162 (Bt-Vip3A) were planted in the Huang-huai-hai summer corn region of China to evaluate the lethal effects on major lepidopteran pests, , , , , , , and using an artificial diet containing lyophilized Bt maize tissue at a concentration representing a 25-fold dilution of tissue.
View Article and Find Full Text PDFPlant Biotechnol J
February 2023
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
China is the world's second-largest maize producer and consumer. In recent years, the invasive fall armyworm Spodoptera frugiperda (J.E.
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