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An improved NRRL YB-4239 genome assembly substantiated by its electrophoretic karyotype.
Microbiol Resour Announc
October 2023
Roy. J. Carver Biotechnology Center, University of Illinois Urbana-Champaign, Urbana, Illinois, USA.
Pacific Biosciences long-read sequencing was used to improve the genome assembly for strain NRRL YB-4239 (ATCC 11503). The new assembly included eight chromosomes that were substantiated by the electrophoretic karyotype. The nuclear genome was 16.
View Article and Find Full Text PDFIntraspecific divergence of diploid grass is associated with structural chromosome changes.
Comp Cytogenet
April 2023
N.I.Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkina str. 3, GSP-1, Moscow 119991, Russia Engelhardt Institute of Molecular Biology, Russian Academy of Sciences Moscow Russia.
Smith in Sibthorp et Smith, 1806 is diploid grass with MM genome constitution occurring mainly in Greece. Two morphologically distinct subspecies - Chennaveeraiah, 1960 and (Holzmann ex Boissier) Eig, 1929 are discriminated within , however, genetic and karyotypic bases of their divergence are not fully understood. We used Fluorescence in situ hybridization (FISH) with repetitive DNA probes and electrophoretic analysis of gliadins to characterize the genome and karyotype of to assess the level of their genetic diversity and uncover mechanisms leading to radiation of subspecies.
View Article and Find Full Text PDFA Chromosome-Level Genome Assembly for Yamadazyma tenuis ATCC 10573.
Microbiol Resour Announc
June 2023
Roy J. Carver Biotechnology Center, University of Illinois Urbana-Champaign, Urbana, Illinois, USA.
Article Synopsis
- PacBio long-read sequencing created a detailed chromosome-level genome assembly for the yeast strain Yamadazyma tenuis ATCC 10573.
- The assembly included 7 chromosomes consistent with the species' electrophoretic karyotype and a 26.5-kb circular mitochondrial genome.
- The total nuclear genome size was 10.8 Mb, had a GC content of 43%, and contained 5,340 predicted genes.
Selection of the methylotrophic yeast Ogataea minuta as a high-producing host for heterologous protein expression.
J Biosci Bioeng
March 2023
Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
Three Ogataea minuta var. minuta strains have been deposited as NBRC 0975, NBRC 10402, and NBRC 10746 in the National Institute of Technology and Evaluation (NITE) Biological Resource Center (NBRC) collection. We investigated the ability to produce secretory proteins and several genotypic and phenotypic characteristics in order to select the best strain for heterologous protein expression.
View Article and Find Full Text PDFDirected Evolution Detects Supernumerary Centric Chromosomes Conferring Resistance to Azoles in Candida auris.
mBio
December 2022
Amity Institute of Integrative Science and Health, Amity University Gurgaon, Gurgaon, Haryana, India.
Candida auris exhibits resistance to multiple antifungal drug classes and sterilization agents, posing threats to the immunocompromised worldwide. Among the four major geographical clades, the East Asian clade 2 isolates of C. auris are mostly drug susceptible.
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