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The [4Fe-4S] cluster is an important cofactor of the base excision repair (BER) adenine DNA glycosylase MutY to prevent mutations associated with 8-oxoguanine (OG). Several MutYs lacking the [4Fe-4S] cofactor have been identified. Phylogenetic analysis shows that clusterless MutYs are distributed in two clades suggesting cofactor loss in two independent evolutionary events.
View Article and Find Full Text PDFConformational changes in polysaccharide-based chiral selectors induced by mobile phase composition: Effects on enantioselective retention and enantiomer elution order reversal.
J Chromatogr A
January 2025
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 10608 Taiwan.
Despite having identical physicochemical properties, chiral molecules require effective separation techniques due to their distinct pharmacological effects. Polysaccharide-based chiral stationary phases (CSPs) are widely used for chiral separations in liquid chromatography; however, the mechanisms of chiral recognition are not well understood. This research explored the adsorption, retention, and chiral recognition mechanisms of three amylose-based CSPs: Chiralpak ID, IF, and IG.
View Article and Find Full Text PDFLameness Recognition of Dairy Cows Based on Compensation Behaviour Analysis by Swing and Posture Features from Top View Depth Image.
Animals (Basel)
December 2024
College of Agricultural Equipment Engineering, Henan University of Science and Technology, Luoyang 471023, China.
Top-view systems for lameness detection have advantages such as easy installation and minimal impact on farm work. However, the unclear lameness motion characteristics of the back result in lower recognition accuracy for these systems. Therefore, we analysed the compensatory behaviour of cows based on top-view walking videos, extracted compensatory motion features (CMFs), and constructed a model for recognising lameness in cows.
View Article and Find Full Text PDFThe Family Doctor in the "COVID-19 Era".
Healthcare (Basel)
December 2024
Internal Medicine I-Discipline of Medical Semiology I, Department V, Center of Advanced Research in Cardiology and Hemostasology, "Victor Babeș" University of Medicine and Pharmacy, Eftimie Murgu Sq. No. 2, 300041 Timisoara, Romania.
The SARS-CoV-2 virus infection, the most severe pandemic in recent human history, found healthcare systems around the world more or less unprepared. Adjusting to this challenge involved changes in the daily routines of healthcare systems, as well as the patients, once again highlighting the importance of primary care (family physician or general practitioner). In the context of the COVID-19 pandemic, the family doctor in Romania played a crucial role in patient management, rapidly adapting to the changes and challenges imposed by the state of emergency.
View Article and Find Full Text PDFEpstein-Barr Virus BRRF1 Induces Butyrophilin 2A1 in Nasopharyngeal Carcinoma NPC43 Cells via the IL-22/JAK3-STAT3 Pathway.
Int J Mol Sci
December 2024
Department of Biology, Faculty of Science, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China.
Epstein-Barr virus is highly associated with nasopharyngeal carcinoma (NPC) with genes expressed for tumor transformation or maintenance of viral latency, but there are certain genes that can modulate immune molecules. Butyrophilin 2A1 (BTN2A1) is an important activating protein for presenting phosphoantigens for recognition by Vγ9Vδ2 T cells to achieve antitumor activities. We have previously shown that Vγ9Vδ2 T cells achieve efficacy against NPC when BTN2A1 and BTN3A1 are upregulated by stimulating EBV gene expression, particularly LMP1.
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