AI Article Synopsis
- - Most bacteria naturally form biofilms, which facilitate important communication processes like quorum sensing (QS), allowing them to gauge cell density and environmental changes.
- - Since QS and biofilms are key to bacterial disease, it's crucial to find non-invasive methods for analyzing these processes in natural bacterial populations.
- - This study uses advanced spectroscopy techniques with specially designed plasmonic substrates to detect QS signaling metabolites in biofilms of Pseudomonas aeruginosa, offering a valuable tool for understanding bacterial communication.
Article Abstract
Most bacteria in nature exist as biofilms, which support intercellular signalling processes such as quorum sensing (QS), a cell-to-cell communication mechanism that allows bacteria to monitor and respond to cell density and changes in the environment. As QS and biofilms are involved in the ability of bacteria to cause disease, there is a need for the development of methods for the non-invasive analysis of QS in natural bacterial populations. Here, by using surface-enhanced resonance Raman scattering spectroscopy, we report rationally designed nanostructured plasmonic substrates for the in situ, label-free detection of a QS signalling metabolite in growing Pseudomonas aeruginosa biofilms and microcolonies. The in situ, non-invasive plasmonic imaging of QS in biofilms provides a powerful analytical approach for studying intercellular communication on the basis of secreted molecules as signals.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082732 | PMC |
| http://dx.doi.org/10.1038/nmat4720 | DOI Listing |
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