In the current study, we present a green toolbox to produce ecological compounds like lactone moiety. Rhodococcus erythropolis DSM 44534 cells have been used to oxidize both decane-1,4-diol () and decane-1,5-diol () into the corresponding γ- () and δ-decalactones () with yield of 80% and enantiomeric excess (ee) = 75% and ee = 90%, respectively. Among oxidation of meso diols, (-)-(1S,5R)-cis-3-oxabicyclo[4.3.0]non-7-en-2-one (5a) with 56% yield and ee = 76% as well as (-)-(2R,3S)-cis-endo-3-oxabicyclo[2.2.1]dec-7-en-2-one (6a) with 100% yield and ee = 90% were formed. It is worth mentioning that R. erythropolis DSM 44534 grew in a mineral medium containing ethanol as the sole source of energy and carbon Chirality 28:623-627, 2016. © 2016 Wiley Periodicals, Inc.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/chir.22623 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Bacterial Lactonases ZenA with Noncanonical Structural Features Hydrolyze the Mycotoxin Zearalenone.
ACS Catal
March 2024
dsm-firmenich Animal Nutrition and Health R&D Center Tulln, Technopark 1, Tulln 3430, Austria.
Zearalenone (ZEN) is a mycoestrogenic polyketide produced by and other phytopathogenic members of the genus . Contamination of cereals with ZEN is frequent, and hydrolytic detoxification with fungal lactonases has been explored. Here, we report the isolation of a bacterial strain, PFA D8-1, with ZEN hydrolyzing activity, cloning of the gene encoding α/β hydrolase ZenA encoded on the linear megaplasmid pSFRL1, and biochemical characterization of nine homologues.
View Article and Find Full Text PDFBioanalytical System for Determining the Phenol Index Based on BS394(pBS216) Bacteria Immobilized in a Redox-Active Biocompatible Composite Polymer "Bovine Serum Albumin-Ferrocene-Carbon Nanotubes".
Polymers (Basel)
December 2022
Federal Research Center «Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences», G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290 Pushchino, Russia.
The possibility of using the microorganisms sp. 7p-81, BS394(pBS216), s67, 5Ap, X5, F5 and DSM 11331 as the basis of a biosensor for the phenol index to assess water environments was studied. The adaptation of microorganisms to phenol during growth was carried out to increase the selectivity of the analytical system.
View Article and Find Full Text PDFFirst determination of DNA virus and some additional bacteria from (sheep ked) in Tibet, China.
Front Microbiol
September 2022
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China.
Article Synopsis
- Sheep keds are common ectoparasites in sheep that cause harm by biting, sucking blood, and potentially spreading various pathogens like helminths and bacteria.
- In a study conducted in Tibet, DNA was extracted from sheep samples, followed by next-generation sequencing to analyze bacterial and viral communities, revealing that Proteobacteria was the most dominant bacterial phylum.
- The study identified various viral families related to vertebrates and insects, with highlights including the presence of African swine fever virus and several poxviruses, while also noting that many of the bacteria detected were newly reported genera.
Evaluation of P450 monooxygenase activity in lyophilized recombinant E. coli cells compared to resting cells.
AMB Express
December 2021
Institute of Biochemistry, Heinrich-Heine University Düsseldorf, Universitätsstraße 1, 40225, Düsseldorf, Germany.
Cytochromes P450 catalyze oxidation of chemically diverse compounds and thus offer great potential for biocatalysis. Due to the complexity of these enzymes, their dependency of nicotinamide cofactors and redox partner proteins, recombinant microbial whole cells appear most appropriate for effective P450-mediated biocatalysis. However, some drawbacks exist that require individual solutions also when P450 whole-cell catalysts are used.
View Article and Find Full Text PDFsp. nov., isolated from a cave, and proposals that is a later synonym of , and are later synonyms of , and and are later synonyms of .
Int J Syst Evol Microbiol
July 2021
Department of Biological Sciences and Biotechnology, Hannam University, Daejon 34054, Republic of Korea.
Two novel actinobacterial strains, designated C9-5 and C3-43, were isolated from soil samples of a cave in Jeju Island, Republic of Korea, and subjected to taxonomic study by a polyphasic approach. The organisms exhibited a typical rod-coccus developmental cycle during growth and grew at 10-30 °C, pH 5-9 and 0-3 % (w/v) NaCl. In 92 single-copy core gene sequence analysis, strain C9-5 was loosely associated with , albeit sharing low 16S rRNA gene sequence similarity (97.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!