AI Article Synopsis
- * The authors proposed a model called the parallel lever arms model to explain how the adjacent binding state allows for this unique stepping motion, with both lever arms oriented in the same direction.
- * To investigate further, researchers created a modified version of myosin VI that replaced a specific part of its structure with a part from another myosin that cannot fold; this modification still produced the same stepping patterns, suggesting that the lever arm extension is not responsible for the adjacent binding state.
Article Abstract
Myosin VI is a processive myosin that has a unique stepping motion, which includes three kinds of steps: a large forward step, a small forward step and a backward step. Recently, we proposed the parallel lever arms model to explain the adjacent binding state, which is necessary for the unique motion. In this model, both lever arms are directed the same direction. However, experimental evidence has not refuted the possibility that the adjacent binding state emerges from myosin VI folding its lever arm extension (LAE). To clarify this issue, we constructed a myosin VI/V chimera that replaces the myosin VI LAE with the IQ3-6 domains of the myosin V lever arm, which cannot fold, and performed single molecule imaging. Our chimera showed the same stepping patterns as myosin VI, indicating the LAE is not responsible for the adjacent binding state.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736786 | PMC |
| http://dx.doi.org/10.2142/biophysics.11.47 | DOI Listing |
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