The results of a comprehensive study of 96 patients after cholecystectomy are provided. The higher sensitivity and informativeness of the 13C-labeled mixed triglyceride breath .test compared with 13C-starch breath test for determining functional pancreatic insufficiency in patients after cholecystectomy in early stages of its formation was set.
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Introduction: Medium-chain fatty acids (MCFAs) have attracted considerable attention for preventing or improving obesity, which is a recognized risk factor for lifestyle-related diseases. Medium- and long-chain triglycerides (MLCTs) are expected to improve the metabolism of ingested long-chain triglycerides (LCTs). However, previous studies have reported mixed results.
View Article and Find Full Text PDFAssessing central carbon metabolism in plants can be challenging due to the dynamic range in pool sizes, with low levels of important phosphorylated sugars relative to more abundant sugars and organic acids. Here, we report a sensitive liquid chromatography-mass spectrometry method for analysing central metabolites on a hybrid column, where both anion-exchange and hydrophilic interaction chromatography (HILIC) ligands are embedded in the stationary phase. The liquid chromatography method was developed for enhanced selectivity of 27 central metabolites in a single run with sensitivity at femtomole levels observed for most phosphorylated sugars.
View Article and Find Full Text PDFIndole-3-acetic acid synthesized through the indole-3-pyruvate pathway promotes Candida tropicalis biofilm formation.
PLoS One
March 2021
Department of Dermatology, Center for Medical Mycology, Case Western Reserve University, Cleveland, Ohio, United States of America.
We previously found that the elevated abundance of the fungus Candida tropicalis is positively correlated with the bacteria Escherichia coli and Serratia marcescens in Crohn's disease patients and the three pathogens, when co-cultured, form a robust mixed-species biofilm. The finding suggests that these three pathogens communicate and promote biofilm formation, possibly through secretion of small signaling molecules. To identify candidate signaling molecules, we carried out a metabolomic analysis of the single-species and triple-species cultures of the three pathogens.
View Article and Find Full Text PDFPrediction of exocrine and endocrine insufficiency after pancreaticoduodenectomy using volumetry.
Acta Chir Belg
August 2020
Department of Hepatobiliary, Endocrine and Transplantation Surgery, Antwerp University Hospital, Edegem, Belgium.
The aim of this study is to evaluate the use of pancreatic volumetric assessment to predict exocrine and endocrine insufficiency after pancreaticoduodenectomy. Thirty-seven patients who underwent pancreaticoduodenectomy were included in the study. Endocrine function was assessed in all patients without a history of diabetes using an oral glucose tolerance test.
View Article and Find Full Text PDFProduction of asymmetric oxidative metabolites of [13C]-β-carotene during digestion in the gastrointestinal lumen of healthy men.
Am J Clin Nutr
October 2018
INRA, INSERM, Aix Marseille University, Marseille, France.
Background: Asymmetric β-apo-carotenoids (nonvitamin A-active metabolites) of provitamin A carotenoids have been observed in humans, but no study has investigated their formation during digestion.
Objective: The aim of this study was to follow the formation and absorption of asymmetric β-apo-carotenoids during digestion.
Design: Healthy men were intragastrically and intraduodenally intubated, and randomly assigned to consume a lipid-rich control meal (n = 3) or a lipid-rich test meal containing 20 mg [13C-10]-β-carotene (n = 7).
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