Keratoconus is an eye disorder that causes the cornea to take an abnormal conical shape, thus impairing its refractive functions and causing blindness. The late diagnosis of keratoconus is among the principal reasons for corneal surgical transplantation. This pathology is characterized by a reduced corneal stiffness in the region immediately below Bowman's membrane, probably due to a different lamellar organization, as suggested by previous studies. Here, the lamellar organization in this corneal region is characterized in three dimensions by means of second-harmonic generation (SHG) microscopy. In particular, a method based on a three-dimensional correlation analysis allows to probe the orientation of sutural lamellae close to the Bowman's membrane, finding statistical differences between healthy and keratoconic samples. This method is demonstrated also in combination with an epi-detection scheme, paving the way for a potential clinical ophthalmic application of SHG microscopy for the early diagnosis of keratoconus. SHG image acquired with sagittal optical sectioning (A) of a healthy cornea and (B) of a keratoconic cornea. Scale bars: 30 μm.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jbio.201600122 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Multiphoton Microscopy Assessment of Healing From Tendon Laceration and Microthermal Coagula in a Rat Model.
Lasers Surg Med
December 2024
James C Wyant College of Optical Sciences, University of Arizona, Tucson, Arizona, USA.
Objectives: To study the healing response of rat Achilles tendon when lacerated or treated with intense therapeutic ultrasound (ITU) via utilization of multiphoton microscopy (MPM) imaging and histology.
Materials And Methods: The right Achilles tendon of each Sprague Dawley rat within a cohort was partially lacerated. 1 to 2 days post-surgery, each rat received ITU treatment of the Achilles tendon on either the right or left leg.
Novel Optical Criteria and Mechanisms of Critical Decline in Liver Regenerative Potential.
Cells
December 2024
Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, Russia.
The most effective method of treating tumors localized in the liver remains resection. However, in the presence of concomitant pathology, the regenerative potential of the liver is significantly reduced. To date, there is insufficient fundamental data on the mechanisms responsible for the disruption of liver regeneration, and there is no effective method for assessing its regenerative potential.
View Article and Find Full Text PDFUse of optical techniques to evaluate the ionizing radiation effects on biological specimens.
J Radiat Res
December 2024
Department of Stem Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Minami-ku, Hiroshima 734-0037, Japan.
Radiation induces various changes in biological specimens; however, the evaluation of these changes is usually complicated and can be achieved only through investment in time and labor. Optical methods reduce the cost of such evaluations as they require less pretreatment of the sample, are adaptable to high-throughput screening and are easy to automate. Optical methods are also advantageous, owing to their real-time and onsite evaluation capabilities.
View Article and Find Full Text PDFRobust Ferroelectricity in Nonstoichiometric 2D AgCrS via Chemical Vapor Deposition.
Small
December 2024
Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
Ferroelectricity in two-dimensional (2D) materials at room temperature has attracted significant interest due to their substantial potential for applications in non-volatile memory, nanoelectronics, and optoelectronics. The intrinsic tendency of 2D materials toward nonstoichiometry results in atomic configurations that differ from those of their stoichiometric counterparts, thereby giving rise to potential ferroelectric polarization properties. However, reports on the emergence of room temperature ferroelectric effects in nonstoichiometric 2D materials remain limited.
View Article and Find Full Text PDFQuantitative fibrosis identifies biliary tract involvement and is associated with outcomes in pediatric autoimmune liver disease.
Hepatol Commun
January 2025
Department of Pediatrics, Division of Gastroenterology, Hepatology, and Nutrition, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.
Article Synopsis
- The study investigates the use of second harmonic generation (SHG) microscopy combined with artificial intelligence to analyze liver fibrosis in children with autoimmune liver disease (AILD) and aims to find prognostic biomarkers for these patients.
- Researchers analyzed liver biopsy slides from 63 patients to evaluate fibrosis patterns and their correlation with clinical outcomes, and found a significant association between certain fibrosis characteristics and increased risk of liver complications and transplantation.
- The findings suggest that SHG and AI can enhance understanding of liver histopathology in AILD, potentially guiding better management and treatment decisions for affected children.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!