AI Article Synopsis
- High-resolution mass spectrometry (HRMS) is increasingly used in discovery workflows, but its adoption in late-stage drug development has been slow.
- Recent reports suggest that while HRMS can produce data suitable for regulated bioanalysis, it previously lacked the sensitivity needed for very low concentration assays (sub ng/ml).
- Advances in HRMS technology, particularly the Q-Exactive series, have improved sensitivity and selectivity, making it a viable option for regulated quantitative bioanalysis.
Article Abstract
High-resolution MS (HRMS) has seen an uptake in use for discovery qual/quan workflows, however, its utilization in late discovery/development has been slow. Past reports comparing HRMS to triple quadrupole (QQQ) instrumentation to date have indicated that HRMS instruments are capable of producing data acceptable for regulated bioanalysis, however lack the sensitivity required for sub ng/ml LLOQ assays. Recent advances in HRMS instrumentation have closed the sensitivity gap with QQQ and have even provided improved selectivity and sensitivity over QQQ SRM assays. Herein, the authors will describe how, when, and why HRMS (specifically Q-Exactive series mass spectrometers) should be considered for implementation in regulated quantitative bioanalysis assays.
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| http://dx.doi.org/10.4155/bio-2016-0079 | DOI Listing |
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