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Ultrastructural demonstration of Cx43 gap junctions in induced pluripotent stem cells from human cord blood. | LitMetric

Ultrastructural demonstration of Cx43 gap junctions in induced pluripotent stem cells from human cord blood.

Histochem Cell Biol

Department of Anatomy and Cell Biology, Saarland University, Kirrberger Straße, Building 61, 66421, Homburg/Saar, Germany.

Published: November 2016

AI Article Synopsis

  • Gap junction proteins, particularly Cx43, are crucial for cell communication and influence stem cell behavior, but their role in undifferentiated stem cells is not well understood.
  • This study focuses on Cx43 expression in human cord-blood-derived induced pluripotent stem cells (hCBiPS2), employing quantitative PCR and immunohistochemistry to analyze their presence.
  • The research reveals that Cx43 is expressed at both mRNA and protein levels in undifferentiated iPS cells, with new ultrastructural data showing the presence of gap junction plaques containing Cx43.

Article Abstract

Gap junction proteins are essential for direct intercellular communication but also influence cellular differentiation and migration. The expression of various connexin gap junction proteins has been demonstrated in embryonic stem cells, with Cx43 being the most intensely studied. As Cx43 is the most prominent gap junction protein in the heart, cardiomyocyte-differentiated stem cells have been studied intensely. To date, however, little is known about the expression and the subcellular distribution of Cx43 in undifferentiated stem cells or about the structural arrangement of channels. We, therefore, here investigate expression of Cx43 in undifferentiated human cord-blood-derived induced pluripotent stem cells (hCBiPS2). For this purpose, we carried out quantitative real-time PCR and immunohistochemistry. For analysis of Cx43 ultrastructure and protein assembly, we performed freeze-fracture replica immunogold labeling (FRIL). Cx43 expression was detected at mRNA and protein level in hCBIPS2 cells. For the first time, ultrastructural data are presented on gap junction morphology in induced pluripotent stem (iPS) cells from cord blood: Our FRIL and electron microscopical analysis revealed the occurrence of gap junction plaques in undifferentiated iPS cells. In addition, these gap junctions were shown to contain the gap junction protein Cx43.

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Source
http://dx.doi.org/10.1007/s00418-016-1469-9DOI Listing

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