A number of reactive dyes coupled to bead cellulose directly or spacer-mediated has been investigated in respect of their interaction with lactate dehydrogenase (LDH; E.C. 1.1.1.28) from heart muscle. The Procion dyes Red HE-7B and Navy H-ER, as well as the Remazol dyes Brilliant Blue R and Brilliant Red 5-BN directly bound to bead cellulose provide high binding of LDH and the adsorbed enzyme is eluted specifically from these affinity adsorbents in high yield. In contrast, under the same conditions no binding of LDH has been found to the Procion dyes Green H-4G, Yellow HE-3G, Scarlet MX-G and Orange MX-G, although an opposite behaviour was expected from the results of affinity partitioning in aqueous two-phase systems. However, immobilizing these dyes via a spacer generated strong binding of the enzyme to the affinity adsorbent. The influence of the length of the spacer was studied in respect of the binding capacity and the yield of the enzyme specifically eluted. The applicability of Procion Scarlet MX-G-(diaminohexyl)-bead cellulose for the purification of LDH from muscle extract in one chromatographic step was demonstrated.
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http://dx.doi.org/10.1016/s0021-9673(01)84627-6 | DOI Listing |
J Mater Chem B
January 2025
College of Materials Science and Engineering, Zhejiang University of Technology, Huzhou 313000, P. R. China.
Traditional natural polysaccharide-based hydrogels, when used as drug carriers, often struggle to maintain long-term stability in the extremely harsh gastric environment. This results in unstable drug release and significant challenges in bioavailability. To address this issue, this study utilized inexpensive and safe natural polysaccharides-chitosan (CS) and high methoxyl pectin (HM)-as raw materials.
View Article and Find Full Text PDFSci Rep
January 2025
Physics Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, 11884, Egypt.
This study aims to synthesize a new localized drug delivery system of bioglass, polyvinyl alcohol (PVA), cellulose (CNC), and sodium alginate (SA) beads as a carrier for methotrexate (MTX) drugs for the treatment of osteosarcoma. Methotrexate /Bioglass-loaded Polyvinyl/Cellulose/Sodium alginate biocomposite beads were prepared via the dropwise method with different concentrations of (65%SiO-30%CaO- 5%PO) bioglass. Samples were named B0, S0, S1, S2, and S3, respectively.
View Article and Find Full Text PDFACS Sustain Chem Eng
January 2025
Department of Chemistry and Biochemistry, Rowan University, Glassboro, New Jersey 08028, United States.
Ethyl cellulose (EC) is a biocompatible, renewable, and recyclable material with diverse sources, making it an attractive candidate for industrial applications. Electrospinning has gained significant attention for the production of EC fibers. However, conventional electrospinning methods face challenges such as bead formation, low yield, and the absence of porous internal structures, limiting both the functional performance and scalability.
View Article and Find Full Text PDFPolymers (Basel)
December 2024
Department of Chemical Engineering, Auburn University, 212 Ross Hall, Auburn, AL 36849, USA.
Cellulose microgel beads fabricated using the dropping technique suffer from structural irregularity and mechanical variability. This limits their translation to biomedical applications that are sensitive to variations in material properties. Ionic salts are often uncontrolled by-products of this technique, despite the known effects of ionic salts on cellulose assembly.
View Article and Find Full Text PDFFood Chem
April 2025
Food and Soft Materials Research Group, Department of Chemistry and Biology, Toronto Metropolitan University, Toronto, Canada. Electronic address:
This study investigated the oleogelation of cellulose bead dispersions in a sunflower oil oleogel made with solvent-transferred whey protein isolate. The microstructure and rheology of the mixed gels depended on the ratio of hydrated cellulose beads to proteins (9:1, 8:2, 7:3, and 1:1). Two gel stabilization mechanisms were identified.
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