AI Article Synopsis
- * A comprehensive resource, the human SRMAtlas, has been created, offering detailed information on 166,174 proteotypic peptides and enabling the identification and quantification of proteins, including their variants and modifications.
- * The SRMAtlas is freely available online and has been applied in research to explore responses to cholesterol synthesis inhibition and docetaxel treatment in liver and prostate cancer cells, showcasing its practical utility.
Article Abstract
The ability to reliably and reproducibly measure any protein of the human proteome in any tissue or cell type would be transformative for understanding systems-level properties as well as specific pathways in physiology and disease. Here, we describe the generation and verification of a compendium of highly specific assays that enable quantification of 99.7% of the 20,277 annotated human proteins by the widely accessible, sensitive, and robust targeted mass spectrometric method selected reaction monitoring, SRM. This human SRMAtlas provides definitive coordinates that conclusively identify the respective peptide in biological samples. We report data on 166,174 proteotypic peptides providing multiple, independent assays to quantify any human protein and numerous spliced variants, non-synonymous mutations, and post-translational modifications. The data are freely accessible as a resource at http://www.srmatlas.org/, and we demonstrate its utility by examining the network response to inhibition of cholesterol synthesis in liver cells and to docetaxel in prostate cancer lines.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245710 | PMC |
| http://dx.doi.org/10.1016/j.cell.2016.06.041 | DOI Listing |
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