Starch is a major energy source for all domains of life. Recent advances in structures of starch-degrading enzymes encompass the substrate complex of starch debranching enzyme, the function of surface binding sites in plant isoamylase, details on individual steps in the mechanism of plant disproportionating enzyme and a self-stabilised conformation of amylose accommodated in the active site of plant α-glucosidase. Important inhibitor complexes include a flavonol glycoside, montbretin A, binding at the active site of human pancreatic α-amylase and barley limit dextrinase inhibitor binding to the debranching enzyme, limit dextrinase using a new binding mode for cereal protein inhibitors.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.sbi.2016.07.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Precision Activity-Based α-Amylase Probes for Dissection and Annotation of Linear and Branched-Chain Starch-Degrading Enzymes.
Angew Chem Int Ed Engl
November 2024
York Structural Biology Laboratory, Department of Chemistry, University of York, York, North Yorkshire, YO10 5DD, UK.
α-Amylases are the workhorse enzymes of starch degradation. They are central to human health, including as targets for anti-diabetic compounds, but are also the key enzymes in the industrial processing of starch for biofuels, corn syrups, brewing and detergents. Dissection of the activity, specificity and stability of α-amylases is crucial to understanding their biology and allowing their exploitation.
View Article and Find Full Text PDFComplete genome sequencing of Enterobacter ludwigii strain T977 revealed its great ability for starch degradation of Nicotiana tabacum L. Yunyan 97.
BMC Microbiol
November 2024
Zhengzhou Tobacco Research Institute of CNTC, Henan Province, Zhengzhou, 450001, PR China.
Enterobacter ludwigii has been proven by numerous studies to be an effective plant growth promoter. Enterobacter ludwigii T977 was isolated from leaves of Nicotiana tabacum L. Yunyan 97 which showing high starch degrading ability.
View Article and Find Full Text PDFAcarbose impairs gut growth by targeting intracellular glucosidases.
mBio
December 2024
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Unlabelled: Acarbose is a type 2 diabetes medicine that prevents dietary starch breakdown into glucose by inhibiting host amylase and glucosidase enzymes. Numerous gut species in the genus enzymatically break down starch and change in relative abundance within the gut microbiome in acarbose-treated individuals. To mechanistically explain this observation, we used two model starch-degrading , (Bo), and (Bt).
View Article and Find Full Text PDFBiotechnological potential of salt tolerant and xerophilic species of Aspergillus.
Appl Microbiol Biotechnol
November 2024
Fungal Physiology, Westerdijk Fungal Biodiversity Institute, Uppsalaan 8, 3584 CT, Utrecht, The Netherlands.
Xerophilic fungi occupy versatile environments owing to their rich arsenal helping them successfully adapt to water constraints as a result of low relative humidity, high-osmolarity, and high-salinity conditions. The general term xerophilic fungi relates to organisms that tolerate and/or require reduced water activity, while halophilic and osmophilic are applied to specialized groups that require high salt concentrations or increased osmotic pressure, respectively. Species belonging to the family Aspergillaceae, and especially those classified in Aspergillus subgenus Aspergillus (sections Restricti and Aspergillus) and Polypaecilum, are particularly enriched in the group of osmophilic and salt-tolerant filamentous fungi.
View Article and Find Full Text PDFSlNAP1 promotes tomato fruit ripening by regulating carbohydrate metabolism.
Plant Physiol Biochem
October 2024
College of Horticulture, Gansu Agricultural University, Lanzhou, 730070, PR China. Electronic address:
Article Synopsis
- * Silencing the SlNAP1 gene resulted in delayed fruit ripening, reduced color change, lower sugar accumulation, and increased starch content in the fruits.
- * Results showed decreased activity of starch-degrading enzymes and lower expression of genes related to both starch and sugar metabolism in SlNAP1-silenced fruits, indicating SlNAP1 may promote fruit ripening by influencing carbohydrate processes.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!