Large-scale image-based screening and profiling of cellular phenotypes.

Cytometry A

Bioinformatics Institute, Agency for Science, Technology and Research, Singapore, 138671, Singapore.

Published: February 2017

Cellular phenotypes are observable characteristics of cells resulting from the interactions of intrinsic and extrinsic chemical or biochemical factors. Image-based phenotypic screens under large numbers of basal or perturbed conditions can be used to study the influences of these factors on cellular phenotypes. Hundreds to thousands of phenotypic descriptors can also be quantified from the images of cells under each of these experimental conditions. Therefore, huge amounts of data can be generated, and the analysis of these data has become a major bottleneck in large-scale phenotypic screens. Here, we review current experimental and computational methods for large-scale image-based phenotypic screens. Our focus is on phenotypic profiling, a computational procedure for constructing quantitative and compact representations of cellular phenotypes based on the images collected in these screens. © 2016 International Society for Advancement of Cytometry.

Download full-text PDF

Source
http://dx.doi.org/10.1002/cyto.a.22909DOI Listing

Publication Analysis

Top Keywords

cellular phenotypes
16
phenotypic screens
12
large-scale image-based
8
image-based phenotypic
8
phenotypic
5
image-based screening
4
screening profiling
4
cellular
4
profiling cellular
4
phenotypes
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!