AI Article Synopsis
- The capsular polysaccharide of Streptococcus pneumoniae serotype 6B was depolymerized using alkaline and acidic conditions, leading to different oligosaccharide products.
- Under alkaline treatment, the major product formed was a specific oligosaccharide structure containing multiple sugar units and a ribose derivative.
- Acidic treatment at lower temperatures produced various oligosaccharides characterized by techniques like sugar analysis, fast atom bombardment-mass spectrometry, and NMR spectroscopy.
Article Abstract
The capsular polysaccharide of Streptococcus pneumoniae serotype 6B [----2)-alpha-D-Galp-(1----3)-alpha-D-Glcp-(1----3)-alpha-L-Rhap-( 1----4)- D-RibOH-(5-P----]n was depolymerised under alkaline (NaOH) and acidic (HF) conditions. The former treatment yielded, as the major component, alpha-2-P-Galp-(1----3)-alpha-Glcp-(1----3)-alpha-Rhap-(1----4)-5- P-RibOH. The latter treatment at -16 degrees gave alpha-Galp-(1----3)-alpha-Glcp-(1----3)-alpha-Rhap-(1----4)-Rib OH-(5-P----2)- alpha-Galp-(1----3)-alpha-Glcp-(1----3)-alpha-Rhap-(1----4)-Rib OH and at 4 degrees gave alpha-Galp-(1----3)-alpha-Glcp-(1----3)-alpha-Rhap-(1----4)-Rib OH. These oligosaccharides were characterised by sugar analysis, f.a.b.-m.s., and 1H- and 13C-n.m.r. spectroscopy.
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| http://dx.doi.org/10.1016/0008-6215(89)80008-4 | DOI Listing |
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