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The roles of serum CXCL16 in circulating Tregs and gastrointestinal stromal tumor cells. | LitMetric

AI Article Synopsis

  • Gastrointestinal stromal tumors (GIST) are the most common type of sarcomas found in the digestive system, and the study investigates the expression of CXCL16 and its receptor CXCR6, which are linked to various cancers but have not been studied in GIST.
  • Researchers used several techniques like immunohistochemistry and Western blotting to find that CXCL16 and CXCR6 protein levels were higher in GIST tissues compared to normal tissues, as well as higher serum levels of CXCL16 and circulating Tregs in GIST patients relative to healthy individuals.
  • The study found that higher levels of CXCL16 and CXCR6 correlate with poorer patient survival; moreover, CXCL16 appears to inhibit T

Article Abstract

Gastrointestinal stromal tumors (GIST) are the most common sarcomas of the digestive system. Abnormal expression of CXCL16 and its sole receptor, CXCR6, has been demonstrated in many cancers. However, no studies have shown the relationship between CXCL16 or CXCR6 expression and GIST. In this study, we detected CXCL16 and CXCR6 expression in GIST patient samples by using immunohistochemistry analysis and Western blot analysis. Serum CXCL16 level was determined by using enzyme-linked immunosorbent assay. Circulating Tregs were isolated by using flow cytometry. MTT assay, cell cycle assay, and transwell assay were used to test the effects of recombinant CXCL16 on Tregs and GIST cells in vitro. The levels of CXCL16 and CXCR6 protein were higher in cancer tissues than in normal tissues. Serum CXCL16 level and circulating Tregs were higher in GIST patients than that in the healthy volunteers. CXCL16, CXCR6, serum CXCL16, and circulating Tregs were significantly associated with a decreased survival time of patients. Relative to control cells, high concentration recombinant CXCL16 treated Tregs and GIST cells exhibited lower proliferation and mobility rates as assessed by MTT assay and transwell assay, respectively. Taken together, CXCL16 was observed to mediate the inhibitory effects in Tregs and GIST cells, and these involved suppression of the MEK/ERK signaling pathway.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4935088PMC
http://dx.doi.org/10.2147/OTT.S105245DOI Listing

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