A novel bioluminescence probe for mercury(II) was obtained on the basis of the distinct deprotection reaction of dithioacetal to decanal, so as to display suitable sensitivity and selectivity toward mercury(II) over other ions with bacterial bioluminescence signal. These experimental results indicated such a probe was a novel promising method for mercury(II) bioluminescence imaging in environmental and life sciences ex vivo and in vivo.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.analchem.6b02200 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Small molecule probes for peroxynitrite detection.
Redox Biochem Chem
December 2024
Department of Biophysics, Medical College of Wisconsin, Milwaukee, United States.
Peroxynitrite (ONOO/ONOOH) is a short-lived but highly reactive species that is formed in the diffusion-controlled reaction between nitric oxide and the superoxide radical anion. It can oxidize certain biomolecules and has been considered as a key cellular oxidant formed under various pathophysiological conditions. It is crucial to selectively detect and quantify ONOO to determine its role in biological processes.
View Article and Find Full Text PDFBioluminescent Probes for the Detection of Superoxide and Nitric Oxide.
ACS Chem Biol
January 2025
Promega Corporation, 2800 Woods Hollow Road, Madison, Wisconsin 53711, United States.
The regulation of reactive oxygen species (ROS) such as superoxide (SO) and nitric oxide (NO) is crucial in biology, influencing metabolism and signaling pathways. Imbalances in these species lead to oxidative stress and various diseases. Traditional methods for measuring SO and NO face challenges in terms of sensitivity and specificity, particularly in complex biological matrices.
View Article and Find Full Text PDFThe Intrinsic Fluorescence of Peptide Self-Assemblies Across pH Levels.
Angew Chem Int Ed Engl
December 2024
Kuang Yaming Honors School, Nanjing University, Nanjing, 210023, China.
The regulation of solution pH on the structural and optical properties of peptide self-assemblies remains a critical yet unresolved issue in peptide research. This study investigates the heptapeptide Ac-IHIHIQI-NH and its intrinsic fluorescence across a range of pH levels, demonstrating that variations in pH lead to significant changes in the morphology of the self-assembled structures. While the position of the fluorescence emission remains constant-due to the stability provided by the hydrogen bonding network of the peptide backbone-the intensity of the fluorescence exhibits a direct correlation with the degree of self-assembly.
View Article and Find Full Text PDFInterference of metal ions on the bioluminescent signal of firefly, Renilla, and NanoLuc luciferases in high-throughput screening assays.
Front Chem
November 2024
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland.
Article Synopsis
- Bioluminescent high-throughput screening (HTS) assays using firefly, Renilla, and NanoLuc luciferases are essential tools in research and drug discovery, but can be affected by various metal ions present in biological and environmental samples.
- A study screened 21 metal ions in three different HTS assays, quantifying their interference with luminescence signals and developing optimized buffer variants for better luciferase performance.
- Findings showed that metal ions significantly impacted bioluminescence signals in a way that correlates with known metal ion-ligand affinities, indicating complex interactions that vary depending on the type of luciferase and assay conditions used.
Leading edge biosensing applications based on AIE technology.
Biosens Bioelectron
March 2025
Biomedical Engineering, School of Medicine, The Chinese University of Hong Kong, Shenzhen, 518172, China; Juxintang (Chengdu) Biotechnology Co., Ltd., Chengdu, 641400, China.
Luminescent materials provide a unique method for biological imaging. Luminescent probes can label molecules of interest and present luminescent signals. Bioluminescence bioimaging has shown great efficacy in environmental, live cell and animal studies.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!