Optical properties of a fluorescence molecule can be drastically changed by surface plasmons excited in neighboring metallic nanostructures. Here we investigated the fluorescence enhancement behavior of fluorescein isothiocyanate (FITC) labeled polystyrene nanoparticles coupled to silver island films (SIFs) via a 15 nm polymethyl methacrylate separation layer theoretically and experimentally. Up to 24-fold fluorescence enhancement was experimentally achieved when the annealing time of the 25 nm Ag films was 50 min, which is in good agreement with the theoretical simulation result based on the finite-difference time-domain method. Furthermore, significant fluorescence spectral distortion on SIFs was also observed compared with samples on glass slides, which is sufficiently related to the scattering properties of SIFs and the lifetimes of FITC.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1364/AO.55.005387 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Application of the Chitooligosaccharides and Fluorescence Polarization Technique for the Assay of Active Lysozyme in Hen Egg White.
Biomolecules
December 2024
Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russia.
This study describes the applicability of the fluorescence polarization assay (FPA) based on the use of FITC-labeled oligosaccharide tracers of defined structure for the measurement of active lysozyme in hen egg white. Depending on the oligosaccharide chain length of the tracer, this method detects both the formation of the enzyme-to-tracer complex (because of lectin-like, i.e.
View Article and Find Full Text PDFBrolucizumab and Platelet Activation and Reactivity.
Curr Eye Res
January 2025
Centre for Ophthalmology, Eberhard-Karls University, Tübingen, Germany.
Purpose: This study explores the potential interaction of brolucizumab with platelets and its effects on platelet activation and reactivity, crucial in retinal vasculitis and retinal vascular occlusion. Safety concerns remain of interest, although brolucizumab showed superior retinal efficacy and reduced injection frequency compared to other licensed anti-VEGF agents.
Methods: Resting and activated platelets of healthy volunteers were pretreated with brolucizumab at the following concentrations 0.
Maternal immune activation by toll-like receptor 7 agonist during mid-gestation increases susceptibility to blood-brain barrier leakage after puberty.
Brain Behav Immun Integr
December 2024
Biomedical Sciences, Colorado State University, Fort Collins, CO, United States.
Maternal immune activation (MIA), a maternal stressor, increases risk for neuropsychiatric diseases, such as Major Depressive Disorder in offspring. MIA of toll-like receptor 7 (TLR7) initiates an immune response in mother and fetuses in a sex-selective manner. The paraventricular nucleus of the hypothalamus (PVN), a brain region that is sexually dimorphic and regulates hypothalamic-pituitary-adrenal (HPA) stress responses, have been tied to stress-related behaviors (i.
View Article and Find Full Text PDFScreening and identification of vascular endothelial cell targeting peptide in gastric cancer through novel integrated in vitro and in vivo strategy.
BMC Cancer
December 2024
Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, 510515, Guangdong, P.R. China.
Purpose: Antiangiogenesis therapy has become a hot field in cancer research. Given that tumor blood vessels often express specific markers related to angiogenesis, the study of these heterogeneous molecules in different tumor vessels holds promise for advancing anti-angiogenic therapy. Previously using phage display technology, we identified a targeting peptide named GX1 homing to gastric cancer vessels for the first time.
View Article and Find Full Text PDFSustained Release of RNA Nanoparticles from Reservoir Implant for Ocular Delivery.
J Pharm Sci
December 2024
Division of Pharmaceutical Sciences, College of Pharmacy, University of Cincinnati, Cincinnati, OH 45267. Electronic address:
Article Synopsis
- RNA nanoparticles show promise for ocular delivery, particularly for chronic eye diseases, using a periocular reservoir implant like an episcleral implant.
- The study aimed to create these implants, test their release performance with model substances and RNA nanoparticles, and apply hindered transport theory to analyze the release rates.
- Findings showed that the release rates depended on both the size of the substances and the implant membrane's characteristics, indicating a potential for extended delivery of RNA nanoparticles through this implantation method.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!