Influence of Stream Bottom Substrate on Retention and Transport of Vertebrate Environmental DNA.

Environ Sci Technol

Department of Biological Sciences, Environmental Change Initiative, University of Notre Dame, Notre Dame, Indiana 46556, United States.

Published: August 2016

AI Article Synopsis

  • eDNA is useful for detecting rare species in streams, but it may originate from upstream locations rather than the sampling point.
  • The study used digital droplet PCR (ddPCR) to detect low eDNA concentrations and compare its residence time and transport to a conservative tracer in a stream.
  • Results showed ddPCR improved detection sensitivity, but eDNA follows a different transport dynamic than the tracer, highlighting the need for a better understanding of eDNA transport mechanisms for effective conservation strategies.

Article Abstract

While environmental DNA (eDNA) is now being regularly used to detect rare and elusive species, detection in lotic environments comes with a caveat: The species being detected is likely some distance upstream from the point of sampling. Here, we conduct a series of seminatural stream experiments to test the sensitivity of new digital droplet PCR (ddPCR) to detect low concentrations of eDNA in a lotic system, measure the residence time of eDNA compared to a conservative tracer, and we model the transport of eDNA in this system. We found that while ddPCR improves our sensitivity of detection, the residence time and transport of eDNA does not follow the same dynamics as the conservative tracer and necessitates a more stochastic framework for modeling eDNA transport. There was no evidence for differences in the transport of eDNA due to substrate type. The relatively large amount of unexplained variability in eDNA transport reveals the need for uncovering mechanisms and processes by which eDNA is transported downstream leading to species detections, particularly when inferences are to be made in natural systems where eDNA is being used for conservation management.

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.est.6b01761DOI Listing

Publication Analysis

Top Keywords

transport edna
12
edna
10
environmental dna
8
residence time
8
conservative tracer
8
edna transport
8
transport
6
influence stream
4
stream bottom
4
bottom substrate
4

Similar Publications

Whole-Exome Sequencing: Discovering Genetic Causes of Granulomatous Mastitis.

Int J Mol Sci

January 2025

Department of Molecular Medicine, Aziz Sancar Institute of Experimental Medicine, Istanbul University, 34093 Istanbul, Türkiye.

Granulomatous mastitis (GM) is a rare, benign, but chronic and recurrent inflammatory breast disease that significantly impacts physical and psychological well-being. It often presents symptoms such as pain, swelling, and discharge, leading to diagnostic confusion with malignancy. The etiology of GM remains unclear, though autoimmune and multifactorial components are suspected.

View Article and Find Full Text PDF

Chronic arsenic exposure affects over 140 million people globally. While arsenic easily crosses the placenta, the specific mechanisms impacting placental immune cell populations and fetal health are unclear. Maternal arsenic exposure is epidemiologically linked to increased infection risk, mortality, and cancer susceptibility in offspring, emphasizing the importance of understanding placentally-mediated immune effects.

View Article and Find Full Text PDF

Over 15 000 species of fishes are found globally in the marine environment and DNA barcodes are used extensively to describe, catalogue, understand and manage this diversity. The dataset outlined here represents a DNA barcode reference library of the mitochondrial cytochrome c oxidase subunit 1 gene (COI) from 9767 voucher specimens (representing at least 2220 species and 288 families) of marine fishes. This publicly available dataset in the Barcode of Life Data System (BOLD) represents 17 years (2005-2022) of barcoding of marine fishes identified from Australian territorial waters.

View Article and Find Full Text PDF

Background: Soil microbes play a vital role in the ecosystem as they are able to carry out a number of vital tasks. Additionally, metagenomic studies offer valuable insights into the composition and functional potential of soil microbial communities. Furthermore, analyzing the obtained data can improve agricultural restoration practices and aid in developing more effective environmental management strategies.

View Article and Find Full Text PDF

Background/objectives: The colonization history of house mice reflects the maritime history of humans that passively transported them worldwide. We investigated western house mouse colonization in the Atlantic region through studies of mitochondrial D-loop DNA sequences from modern specimens.

Methods: We assembled a dataset of 758 haplotypes derived from 2765 mice from 47 countries/oceanic archipelagos (a combination of new and published data).

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!