AI Article Synopsis
- Researchers developed fluorescent calcium-selective nanospheres to visualize intracellular calcium levels in different light spectra.
- These nanospheres are made with specific chromoionophores that allow for adjustable fluorescence detection, making it easier to track calcium changes inside cells.
- The nanospheres respond quickly (in about 1 second) to changes in calcium levels and can provide continuous imaging for up to 24 hours, demonstrating their potential for biological studies.
Article Abstract
Continuous fluorescence imaging of intracellular ions in various spectral ranges is important for biological studies. In this paper, fluorescent calcium-selective nanospheres, including calix[4]arene-functionalized bodipy (CBDP) or 9-(diethylamino)-5-[(2-octyldecyl)imino]benzo[a]phenoxazine (ETH 5350) as the chromoionophore, were prepared to demonstrate intracellular calcium imaging in visible or near-IR regions, respectively. The fluorescence of the nanospheres was controlled by the chromoionophore, and thus the spectral range for detection was adjustable by choosing the proper chromoionophore. The response time of the nanospheres to calcium was typically 1 s, which allowed accurate measurement of intracellular calcium. These nanospheres were loaded into cells through free endocytosis and exhibited fluorescence for 24 h, and their intensity was correlated with the elevation of intracellular calcium upon stimulation. The successful demonstration of calcium imaging by use of ion-selective nanospheres within two spectral ranges in 24 h supported that these nanospheres could be applied for continuous imaging of intracellular ions with adjustable spectra.
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| http://dx.doi.org/10.1021/acsami.6b05406 | DOI Listing |
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