AI Article Synopsis
- A new method using magnetic nanoparticles was developed to immobilize lipase in organic solvents, with toluene proving to be the most effective solvent for this process.
- The maximum immobilization yield reached 97%, and the immobilized lipase showed a relative activity of 124% at an optimal temperature of 30 °C.
- The lipase maintained 67% residual activity after 10 uses, indicating benefits like easy recovery, high stability, and increased activity, which could be valuable for industrial applications in anhydrous environments.
Article Abstract
In order to improve the performance of lipase in organic solvents, a simple immobilization method was developed by adsorption of lipase onto Fe₃O₄@ SiO₂magnetic nanoparticles in organic solvent. Among the solvents tested, toluene was found to be the most effective solvent for the immobilization. A maximum immobilization yield of 97% and relative activity of 124% were achieved in toluene at 30 °C. The optimal temperature, enzyme loading and water activity were 30 °C, 1.25 mg/mg support and 0.48 aw, respectively. The residual activity of immobilized lipase was 67% after 10 cycles of use. The advantages of the immobilized lipase including easy recovery, high stability, and enhanced activity of immobilized lipase in organic solvents show potential industrial applications in anhydrous solvents.
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| http://dx.doi.org/10.1166/jnn.2016.10694 | DOI Listing |
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