AI Article Synopsis
Article Abstract
Cytoplasmic actin and actin-associated proteins have been identified in RABV particles. Although actin is involved in RABV entry into cells, the specific role of actin in RABV budding and release remains unknown. Our study found that RABV M protein-mediated virion budding depends on intact actin filaments. Confocal microscopy demonstrated a block to virions budding, with a number of M protein-mediated budding vesicles detained in the cell cytoplasm. Furthermore, RABV infection resulted in inactivation of cofilin and upregulation of phosphorylated cofilin. Knockdown of cofilin reduced RABV release. These results for the first time indicate that RABV infection resulted in upregulation of phosphorylated cofilin to facililtate actin polymerization for virus budding.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bbrc.2016.07.030 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Young Hearts: A Film Review.
J Homosex
January 2025
College of Nursing, National Taipei University of Nursing and Health Sciences (NTUNHS), Taipei, Taiwan.
Young Hearts, a gay movie from Belgium directed by Anthony Schatteman, was released in December 2024. It offers a tender and nuanced portrayal of queer love, identity, and self-discovery. The film follows Elias and Alexander, two 14-year-olds navigating the complexities of a budding relationship amidst societal expectations in a conservative town.
View Article and Find Full Text PDFVirulence expression difference to intestinal cells of different pathogenic Listeria monocytogenes contaminating sausages after simulated digestive tract.
Int J Food Microbiol
January 2025
State Key Laboratory of Meat Quality Control and Cultured Meat Development, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, PR China. Electronic address:
This study investigated the difference in survival among Listeria monocytogenes (LM) 10403S (highly pathogenic strain) and M7 (low pathogenic strain) in sausage under a simulated digestive environment, and established intestinal organoids and macrophages co-culture model to further explore the virulence expression difference to intestinal cells between LM 10403S and M7 after in vitro gastrointestinal digestion. Results showed that, compared with LM M7, LM 10403S exhibited a high survival rate during in vitro digestion, which may be due to the increased expression of stress response-related genes. In addition, the expression of virulence genes in LM 10403S was significantly higher than in LM M7 under the gastrointestinal environment.
View Article and Find Full Text PDFAssessment of SREBP Activation Using a Microsomal Vesicle Budding Assay.
Bio Protoc
December 2024
Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Sterol regulatory element binding proteins (SREBPs) are transcription factors that reside in the endoplasmic reticulum (ER) membrane as inactive precursors. To be active, SREBPs are translocated to the Golgi where the transcriptionally active N-terminus is cleaved and released to the nucleus to regulate gene expression. Nuclear SREBP levels can be determined by immunoblot analysis; however, this method can only determine the steady-state levels of nuclear SREBPs and does not capture the actual status of activation.
View Article and Find Full Text PDFExploring the druggability of the UEV domain of human TSG101 in search for broad-spectrum antivirals.
Protein Sci
January 2025
Department of Physical Chemistry, Institute of Biotechnology, and Unit of Excellence in Chemistry Applied to Biomedicine and Environment, School of Sciences, University of Granada, Granada, Spain.
The ubiquitin E2 variant domain of TSG101 (TSG101-UEV) plays a pivotal role in protein sorting and virus budding by recognizing PTAP motifs within ubiquitinated proteins. Disruption of TSG101-UEV/PTAP interactions has emerged as a promising strategy for the development of host-oriented broad-spectrum antivirals with low susceptibility to resistance. TSG101 is a challenging target characterized by an extended and flat binding interface, low affinity for PTAP ligands, and complex binding energetics.
View Article and Find Full Text PDFRecent Advances in Microfluidics for Nucleic Acid Analysis of Small Extracellular Vesicles in Cancer.
Adv Healthc Mater
December 2024
School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW, 2007, Australia.
Small extracellular vesicles (sEVs) are membranous vesicles released from cellular structures through plasma membrane budding. These vesicles contain cellular components such as proteins, lipids, mRNAs, microRNAs, long-noncoding RNA, circular RNA, and double-stranded DNA, originating from the cells they are shed from. Ranging in size from ≈25 to 300 nm and play critical roles in facilitating cell-to-cell communication by transporting signaling molecules.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!