AI Article Synopsis
- Identification of nematode species typically involves fixing the isolated specimens and preparing them for analysis, often starting with microscopic identification.
- Various preparation methods for nematodes from the genus Aphelenchoides are documented, emphasizing the need for appropriate fixation techniques.
- Common fixatives used in these processes include formalin, FAA (Formalin-Acetic Acid-Alcohol), and TAF (Triethanolamine-Formalin), as noted in multiple studies over the years.
Article Abstract
Identification of nematode species by using conventional methods requires fixation of the isolated material and a suitable preparation for further analyses. Tentative identification using microscopic methods should also be performed prior to initiating molecular studies. In the literature, various methods are described for the preparation of nematodes from the genus Aphelenchoides for identification and microscopic studies. The most commonly used fixatives are formalin (Timm 1969; Szczygieł & Cid del Prado Vera 1981, Crozzoli et al. 2008, Khan et al. 2008), FAA (Wasilewska 1969; Vovlas et al. 2005, Khan et al. 2007) and TAF (Hooper 1958, Chizhov et al. 2006, Jagdale & Grewal 2006).
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| http://dx.doi.org/10.11646/zootaxa.4083.2.9 | DOI Listing |
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