Laccases are multicopper oxidoreductases with broad substrate specificity and are applied in biofuel cells at the cathode to improve its oxygen reduction performance. However, the production of laccases by e.g. fungi is often accompanied by the need of synthetic growth supplements for increased enzyme production. In this study we present a strategy for the white-rot fungus Pleurotus sajor-caju for natural laccase activity induction using lignocellulose substrates and culture supernatant of Aspergillus nidulans. P. sajor-caju laccases were secreted into the supernatant, which was directly used at a carbon-nanotube buckypaper cathode in a biofuel cell. Maximal current densities of -148±3μAcm(-2) and -102±9μAcm(-2) at 400mV were achieved at pH 5 and 7, respectively. Variations in cathode performance were observed with culture supernatants produced under different conditions due to the induction of specific laccases.
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| http://dx.doi.org/10.1016/j.biortech.2016.06.126 | DOI Listing |
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Article Synopsis
- Scientists studied how certain types of mushrooms, like Pleurotus, can help break down harmful antibiotics in water, which can happen when waste isn't thrown away properly.
- They found that the laccase enzyme from these mushrooms can degrade antibiotics like levofloxacin very effectively, with one type of mushroom breaking down up to 77.7% of it in 25 days.
- The researchers used tests to compare the mushroom extracts with a commercial enzyme and found that the mushrooms were almost just as good at removing the antibiotics, helping protect the environment from these harmful substances.
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